摘要
本试验旨在研究壳寡糖对花鲈(Lateolabrax japonicus)幼鱼生长,肠道消化,血清生化指标及肠道菌群的影响。本试验选用360尾规格一致的花鲈幼鱼(19.37g左右)为研究对象。壳寡糖饲料添加浓度设为6组:0%、0.2%、0.4%、0.6%、0.8%、1.0%。设3个平行组,养殖周期45d。结果显示:(1)生长性能:添加0.6%和0.8%两组的增重率、特定生长率与对照组相比有提高(P>0.05);(2)肠道消化酶:添加0.6%~1.0%的壳寡糖显著(P<0.05)提高胰蛋白酶活性,0.4%~0.8%的壳寡糖显著(P<0.05)提高了脂肪酶活性;(3)肠道菌群:0.6%~1.0%的壳寡糖显著降低了沙门氏菌数量(P<0.05),大肠杆菌的数量在0.8%时显著降低(P<0.05),双歧杆菌的数量在0.2%~0.6%时显著增加(P<0.05);(4)血清生化指标:壳寡糖显著提高血清总蛋白和高密度脂蛋白胆固醇含量(P<0.05);(5)血清非特异性免疫:添加0.6%的壳寡糖显著降低丙二醛(P>0.05),0.2%的壳寡糖增强超氧化物歧化酶、碱性磷酸酶和过氧化物酶活性,添加壳寡糖使溶菌酶的活性显著增强(P<0.05)。研究结果表明:饲料中添加0.6%~0.8%的壳寡糖对花鲈幼鱼生长性能、血脂及肠道健康影响效果最佳。
This research was conducted to evaluate the effects of dietary supplementation with Chitosan-oligosaccharide（COS）on the growth performance,digestion,serum biochemical indexes and intestinal flora.A total of 360 juvenile Japanese seabass were randomly assigned to six treatment diets that included COS at concentrations of0%,0.2%,0.4%,0.6%,0.8%,1.0%for 45 d.Each treatment had 3 replicates with 20.The results showed：（1）The growth performance：The weight gain and specific growth rate of Diet3（0.6%）and Diet4（0.8%）were increased compared with the control group（P〈0.05）;（2）The digestive ferment：The intestinal trypsin activity were significantly increased（P〈0.05）in the Diet3（0.6%）,Diet4（0.8%）and Diet5（1.0%）.Intestinal lipase activity was remarkably increased at Diet2（0.4%）,Diet3（0.6%）and Diet4（0.8%）（P〈0.05）.There was no significant difference in intestinal amylase activity（P〈0.05）.（3）The serum biochemical indexes：Compared with the control group,the contents of the serum total protein and high-density lipoprotein cholesterol（HDL-C）was obviously increased（P〈0.05）;（4）The nonspecific immunity：The contents of MDA was decreased significantly（P〈0.05）on the Diet3（0.6%）.The activities of superoxide dismutase,alkaline phosphatase and peroxidase were all increased（P〈0.05）on the Diet1（0.2%）,and the serum lysozyme was significantly increased（P〈0.05）.（5）Intestinal flora：The number of Salmonella in Diet3（0.6%）,Diet 4（0.8%）and Diet5（1%）,and E.coli was significantly decreased（P〈0.05）in Diet（0.8%）.The quantity of bifidobacteria increased significantly（P〈0.05）in Diet1（0.2%）,Diet2（0.4%）and Diet3（0.6%）.In conclusion,these results indicate that COS can enhance growth and immunity of juvenile Japanese seabass,and the optimum addition level was 0.6%~0.8%in diet.
出处
《海洋学报》
CAS
CSCD
北大核心
2018年第2期69-76,共8页
基金
福建省科技重点项目(2015N0010)
厦门市科技项目(3502Z20143017)
关键词
花鲈
壳寡糖
生长性能
非特异性免疫
肠道菌群
Lateolabrax japonicas
chitosan-oligosaccharide
growth performance
non-specific immune
intestinal flora
