摘要
目的探讨鼻咽癌易感TERT基因多种靶miRNA在鼻咽癌患者血清中含量对临床诊断的价值。方法收集在本医院就诊60例鼻咽癌者及60例健康者外周血,RT-PCR法检测外周血中mir-138、mir-491、mir-181、mir-1207、mir-346、mir-512-5P、mir-299、mir-1266、mir-1182相对表达量差异,分析mir-1182含量与鼻咽癌患者临床病理特征相关性。结果鼻咽癌患者血液中mir-346、mir-512-5P、mir-299、mir-1266、mir-1182含量低于健康对照组,mir-1182差异显著(P<0.01);Ⅲ~Ⅳ期、肿瘤直径≥5 cm及癌转移者血清中mir-1182含量分别低于Ⅰ~Ⅱ期、肿瘤直径≤5 cm及无转移患者(P<0.05);mir-1182诊断AUC为0.851(95%CI:0.782~0.919),灵敏度65%,特异度93.3%。结论鼻咽癌患者血清中mir-1182含量与肿瘤大小、有无淋巴结转移、TNM分期、病理分期负相关,可见mir-1182有较好诊断效能,具有鼻咽癌临床诊断运用价值。
OBJECTIVE To investigate the value of multiple target miRNAs of nasopharyngeal carcinoma（NPC） susceptible gene TERT in the serum of patients with NPC for clinical diagnosis. METHODS The peripheral venous blood from 60 paitents with NPC and 60 healthy volunteers in our hospital were collected., The relative expression of miR-138, mir-491, miR-181, mir-1207, mir-346, mir-512-5P, mir-299, mir-1266 and mir-l182 of peripheral venous blood was detemined by RT-PCR, and the correlation between mir- 1182 expression levels and clinical pathological features of patients with NPC was analyzed. RESULTS The expression levels of mir-1207, mir-346, mir-512-5P, mir-299, mir-1266 andmir-1182 of the peripheral venous blood in patients with NPC were lower than those in healthy people, with a significant difference observed for mir-1182（P〈0.01）. The expression levels of mir-1182 in patients during Ⅲ-Ⅳ phase, in the tumors which diameter was greater than or equal to 5 cm and in the serum of patients with cancer metastasis were respectively lower than those during Ⅰ-Ⅱ phase, in the tumors which diameter was less than or equal to 5 cm and in the serum of patients with no cancer metastasis（P〈0.05）. The diagnostic value of AUC for mir-1182 was 0.851（95%CI： 0.782-0.919）, with both the sensitivity and the specificity was 65% and 93.3% respectively. CONCLUSION There is a negative correlation between the serum expression levels in the patients with NPC and thetumor size, lymph node metastasis, TNM phase and pathological staging, indicating that mir-1182 exerts abetter diagnostic performance and contributes to therapeutic strategies for NPC.
出处
《中国耳鼻咽喉头颈外科》
CSCD
2018年第1期13-16,共4页
Chinese Archives of Otolaryngology-Head and Neck Surgery
