摘要
目的 :初步探讨全口义齿修复对口腔微生态的影响。方法 :选取11例全口义齿修复后7 d的患者,收集口腔不同软组织位点、唾液及义齿表面的菌斑,构建细菌16S r RNA基因文库。采用焦磷酸测序技术针对细菌的16S r RNA基因序列的V2-V3可变区进行序列分析,完成口腔菌群的物种鉴定。结果:11例全口义齿修复患者共测得64800条序列,其中37416条序列为缓症链球菌、溶血孪生球菌、黏液罗氏菌、卟啉单胞菌、动物咬伤奈瑟球菌、苛养颗粒链菌、鲍曼不动杆菌、香茅醇假单胞菌、毗邻颗粒链菌和梭杆菌,为口腔优势菌种。全口义齿组织面的物种与唇颊前庭区和口底菌种较为相似,而全口义齿光滑面的物种与口底及舌腹区黏膜菌种的相似度较高。结论:全口义齿修复对口腔菌群的影响仍有一定局限性,口腔菌群组成不同还受到个体差异等多因素影响。
PURPOSE： This study investigateed the influence of complete denture on oral bacteria flora. METHODS：Bateria plaque samples in oral mucosa, saliva and denture surfaces in 11 edentulous patients were collected, then the 16 S r RNA gene clone libraries were constructed. Pyrosequencing was used to analyze the 16 S rRNA gene V3~V4 regions, oral bacteria flora were classified and identified. RESULTS： There were 64800 sequences in complete denture-wearing subjects, Streptococcus mitis, Gemella haemolysans, Rothia mucilaginosa, Porphyromonas sp, Neisseria zoodegmatis,Granulicatella elegans, Acinetobacter baumannii, Pseudomonas citrinum, Granulicatella adiacens and Fusobacterium canifelinum were the predominant species（37416 sequences）. The species of denture tissue surface were similar to these of buccal vestibule after wearing denture, and the species of denture smooth surface were similar to these of tongue ventrum and the floor of mouth. CONCLUSIONS： The effect of complete denture on oral flora is still limited, and the composition of oral flora is influenced by many other factors.
出处
《上海口腔医学》
CAS
CSCD
北大核心
2018年第1期56-60,共5页
Shanghai Journal of Stomatology
基金
上海市卫生局项目(2012173)
