摘要
将参与柿果实低氧胁迫响应的异源基因DkERF9转化到切花菊‘九月九’植株中,经PCR检测,获得了5株过量表达(35S::DkERF9)的阳性植株。进一步比较分析了35S::DkERF9植株与野生型植株的耐淹水性,发现水培低氧胁迫处理2周后,35S::DkERF9植株较野生型植物生长缓慢,根系生长和茎的伸长均受到显著抑制,叶片颜色较淡;移除水培低氧胁迫处理3周后,生长部分恢复,但是叶片颜色及整体长势均弱于野生型植株。
The exogenous DkERF9,a reported hypoxia responsive gene from persimmon,was transformed into‘Jiuyuejiu’chrysanthemum.Five positive lines(35 S::DkERF9)were obtained and verified with PCR.Compared to the wild type plants,the growth of 35 S::DkERF9 lines were inhibited significantly by water planting treatments for two weeks,as the length of roots and shoots were shorter in 35 S::DkERF9 lines.The differences between 35 S::DkERF9 lines and wild type plants remain significant,after three weeks recovery.
作者
刘晓芬
向理理
殷学仁
李方
陈昆松
LIU Xiaofen;XIANG Lii;YIN Xueren;LI Fang;CHEN Kunsong(Zhejiang Provincial Key Laboratory ofHorticultural Plant Integrative Biology,College of Agriculture&Biotechnology,Zhejiang University,Hangzhou 310058,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2018年第1期109-116,共8页
Acta Horticulturae Sinica
基金
“十二五”农村领域国家科技计划课题(2013AA102700)
国家自然科学基金青年基金项目(31401909)
曹光彪高科技发展基金项目
关键词
菊花
ERF
涝害
低氧胁迫
遗传转化
chrysanthemum
ERF
waterlogging
hypoxia
transformation
