摘要
对‘嘎拉’苹果中的1个bZIP转录因子基因MdAREB2(序列号:MDP0000248567)进行功能初步研究。蛋白进化树分析表明,苹果MdAREB2与拟南芥AtAREB2具有很高的同源性。利用PlantCare数据库进行启动子顺式作用元件的预测分析,MdAREB2启动子序列中存在脱落酸响应元件ABRE。实时定量PCR检测表明,MdAREB2明显受ABA诱导。拟南芥种子萌发阶段经过0.5和2μmol·L^(-1)ABA处理后发现突变体abi5中过量表达MdAREB2能够恢复对ABA的敏感性。拟南芥幼苗生长阶段,经过20μmol·L^(-1)ABA处理后发现,突变体abi5中过量表达MdAREB2能够部分恢复对ABA的敏感性。
A bZIP transcription factor gene MdAREB2(MDP0000248567)was cloned from‘Gala’apple(Malus×domestica Borkh.).The evolutionary tree analysis indicated that MdAREB2 had the very high homology with AtAREB2.The promoter of Md AREB2 was analyzed and predicted by Plant Care databases,and the results showed that the promoter sequence contained abscisic acid response element ABRE.Quantitative real-time PCR showed that MdAREB2 was significantly induced by ABA.This study found that with 0.5μmol·L^(-1)ABA and 2μmol·L^(-1)ABA treatment,excessive expression of MdAREB2 in mutant abi5 could restore its sensitivity to ABA in seed germination stage.In the seedling growth stage,with 20μmol·L^(-1)ABA treatment,excessive expression of MdAREB2 in mutant abi5 could partially restore its sensitivity to ABA.
作者
刘亚静
马齐军
路静
郝玉金
由春香
LIU Yajing;MA Qijun;LU Jing;HAO Yujin;YOU Chunxiang(State Key Laboratory of Crop Biology,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops inHuanghuai Region,Ministry of Agriculture,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an,Shandong 271018,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2018年第1期1-10,共10页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31471854)
农业部“948”国际交流项目子课题(2016-X11)
