摘要
i小尾寒羊经乳房实验性感染大肠杆菌，利用半定量RTPCR方法检测大肠杆菌感染后不同时间小尾寒羊外周血白细胞中TLR1～5基因mRNA的表达情况。结果显示，大肠杆菌感染后外周血白细胞中TLR1～5基因mRNA的表达均出现先升高后降低的趋势。其中TLR3、TLR4和TLR5基因的相对表达量于大肠杆菌感染后24，36，48h均出现显著或极显著升高，而TLR2和TLR4基因的相对表达量于感染后72h时出现显著降低。结果表明，大肠杆菌感染后，TLR3、TLR4和TLR5受体与细菌的识别、炎症的发生密切相关，为进一步研究TLR1～5基因在绵羊大肠杆菌乳房炎发病过程中的作用机制奠定了基础。
Small-tail Han sheep was experimentally infected with E. coli through beast and semi- quantitative RT-PCR method was adopted to detect the expression of mRNA of peripheral blood leukocytes after the infection at different times. The results showed that the expression of TLR1-5 mRNA of E. coil-affected peripheral blood leukocytes increased first and then decreased. The rela- tive expression of TLR3, TLR4, TLR5 genes increased significantly or extremely significantly after 24,36,48 h and TLR2,TLR4 gene relative expression decreased markedly after 72 h. This reveals that TLR3,TLR4,TLR5 receptors closely relate to bacteria identification and inflammation after the E. coli infection. This study lays the groundwork for the future research of TLR1-5 in the pathogenesis of E. coli mastitis.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第2期281-286,共6页
Chinese Journal of Veterinary Science
基金
甘肃省高等学校基本科研业务费资助项目
甘肃省农业生物技术研究与应用开发资助项目(GNSW-2011-22)
关键词
小尾寒羊
大肠杆菌
TLR1~TI
R5
半定量RT-PCR
small tail Han sheep
E. coli
Toll-like receptor 1-5 （TLR1-5）
semi-quantitative reverse transcription polymerase chain reaction（RT-PCR）
