摘要
为了解猪流行性腹泻病毒（PEDV）、猪轮状病毒（PoRV）、猪传染性胃肠炎病毒（TGEV）在我国原种猪场的流行情况，采用ELISA检测方法对2016年1—6月采集的896份发病种猪血清样品进行了血清学检测。结果显示，PEDV、PoRV、TGEV抗体阳性率分别为62．3％，89．0％，23．0％，且存在混合感染情况，其中PEDV＋PoRV、PEDV＋TGEV、PoRV＋TGEV混合感染率分别为42．3％，8．5％，5．6％，PEDV＋PoRV＋TGEV混合感染率为8．9％；同时对检测样品进行了不同种猪类别的比较和不同日龄段的比较，发现不同类别种猪抗体阳性率和不同目龄种猪抗体阳性率的分布特征显著。结果说明我国原种猪场3种腹泻病毒中PoRV感染率最高，其次是PEDV和TGEV，且在不同种猪类别和不同日龄段的种猪中呈现规律性分布。本试验对国内原种猪场猪血清进行PEDV、PoRV和TGEV抗体阳性率检测，为我国种猪病毒性腹泻的防控提供了参考。
To investigate the prevalence of porcine epidemic diarrhea us virus（PoRV） and transmissible gastroenteritis virus （TGEV） in virus（PEDV）, porcine rotavir- breeding pig farm of China,a total of 896 serum samples were collected from January 2016 to June 2016 and ELISA methods were used to detect the antibodys against related viruses. The result showed that the positive rates of the antibodys against PEDV,PoRV and TGEV were 62.3% ,89.0% and 23.0% ,respectively. The mixed infection rate of PEDV ＋PoRV, PEDV ＋TGEV and PoRV ＋TGEV were 42. 3%, 8.5% and 5.6% respectively and PEDV＋PoRV＋TGEV were 8.9%. Types and space analysis of the samples showed that they were distributed regularly. The result showed that the PoRV infec- tion rate was higher than that of PEDV and TGEV in pig breeding farms. It provides reference in- formation for the prevention and control of swine virus diarrhea.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第2期255-258,共4页
Chinese Journal of Veterinary Science
基金
科技部科技基础性工作专项动物及动物产品质量安全检测标准物质研制资助项目(2013FY113300-6)
关键词
种猪
猪流行性腹泻病毒
猪轮状病毒
猪传染性胃肠炎病毒
血清学检测
swine
porcine epidemic diarrhea virus
porcine rotavirus virus
transmissible gastroenteritis virus
serological detection
