摘要
金纳米簇(Au NCs)具有拟过氧化物酶活性,能催化鲁米诺(Luminol)与H_2O_2反应,产生增强的化学发光信号。不同于其它纳米微粒催化的Luminol-H_2O_2化学发光反应,Au NCs催化得到的是慢化学发光信号,且其信号能在10min内保持稳定。基于此反应,本文发展了简单、方便的化学发光测定H_2O_2的新方法。在优化的实验条件下,测定H_2O_2的检测限为10μmol/L。将此方法应用于葡萄糖的检测,其线性范围为10~1 000μmol/L,检测限为10μmol/L。目前一些重要的生物分子,如尿酸和乳酸等均能与相关酶反应生成H_2O_2,本方法也能进一步拓宽至这些生物分子的检测。
Gold nanoclusters（Au NCs） which exhibit peroxidase mimic activity can catalyze the reaction between luminol and H2 02, resulting an enhanced chemiluminescence signal. Different from other nanoparticles catalyzed luminol-H2 02 chemiluminescence reactions, a slow chemiluminescence signal would be obtained by using Au NCs as catalyst in luminol-H2 02 chemiluminescence reaction, and the chemiluminescence would maintain more than 10 rain without decay. Based on the above Au NCs catalyzed chemiluminescence reaction,a simple chemiluminescence method for the detection of H2 02 was developed. Under optimum experimental conditions, the detection limit for H2 02 was 10 μmol/L. The proposed method was then applied in the detection of glucose. The linear range of glucose detection was 10-1000 μumol/L with a detection limit of 10 μmol/L. The results demonstrate the potential application of the proposed method in determination of enzyme-coupled catalyzed biologically important molecules, such as uric acid and lactic acid.
出处
《分析科学学报》
CAS
CSCD
北大核心
2018年第1期47-51,共5页
Journal of Analytical Science
基金
国家自然科学基金(No.21475094)
关键词
金纳米簇
H2O2
葡萄糖
鲁米诺
慢化学发光
Gold nanocluster
Hydrogen peroxide
Glucose
Luminol
Slow chemiluminescence
