摘要
利用99对SSR荧光标记对102份国内蚕豆育成品种(品系)和优异种质资源进行遗传多样性分析。结果表明:99对SSR标记共检测出937个等位变异,每对标记平均检测出4~19个等位变异,平均为9.46个;多态性信息量(PIC)变化范围为0.38~0.88,平均为0.63;有效等位变异(Ne)变化范围为1.79~9.22,平均为3.41;Shannon信息指数变化范围为0.79~2.44,平均为1.45。基于邻接法(NJ)的聚类分析将102份国内育成蚕豆品种(品系)及优异种质划分成春播和秋播两大生态类型;优异蚕豆资源的遗传多样性最高,其次为青海品种(品系)和云南品种,江苏品种遗传多样性相对较低。群体遗传结构和主成分分析将供试蚕豆材料分为三大类,第一大类主要以云南育成品种为主,第二大类全部为青海育成品种(品系),第三大类主要为大部分优异种质资源以及全部江苏育成品种;供试材料具有生态适应性狭窄和极强的地域生态特点。
In order to effectively excavate and utilize faba bean resources,the genetic diversity of 102 varieties(lines)and elite collections of faba bean in China were analyzed by SSR markers.The results indicated that 937 alleles were detected with 9.46 alleles per locus ranging from 4 to 19.The polymorphism information content(PIC)ranged from 0.38 to 0.88 with the mean value of 0.63.The effective number of alleles ranged from 1.79 to 9.22 with the mean value of 3.41.The Shannon index ranged from 0.79 to 2.44 with the mean value of 1.45.The 102 accessions were clustered into spring sowing ecological type and autumn sowing ecological type according to the genetic distances based on neighbor-joining method.The genetic diversity of faba bean varieties(lines)from different origins and elite collections showed that elite collections > Qinghai varieties(lines)> Yunnan varieties > Jiangsu varieties.The population genetic structure and principal component analysis showed that all the accessions were divided into 3 subgroups associated with characteristics of the narrow ecological adaptability and the strong regional ecology.Three subgroups were subgroup 1 including varieties from Yunnan and a small number of elite collections;subgroup 2 including all varieties(lines)from Qinghai;subgroup 3 including major elite collections and all bred varieties from Jiangsu.These results have provided important references for breeding new varieties and germplasm innovation of faba bean.
出处
《中国蔬菜》
北大核心
2018年第2期34-41,共8页
China Vegetables
基金
国家食用豆产业技术体系项目(CARS-09)
国家农作物种质资源共享服务平台项目(NICGR2016-食用豆子平台)
欧盟
北美及大洋洲农业生物资源引进与农业技术需求和政策信息收集研究项目(2016-X16)
关键词
SSR标记
蚕豆
品种
优异种质
遗传多样性
SSR marker
Faba bean(Vicia faba L.)
Variety
Elite collections
Genetic diversity
