摘要
细胞的氧化还原状态或活性氧水平是其基本的生理特征。它自身受到精细的调控,同时也调控细胞功能,在细胞、机体的生理和病理活动中扮演重要角色。检测细胞氧化还原状态及其变化是目前基础研究和临床研究中的重要内容。该文就目前检测方法中的荧光探针做一综述。通过介绍不同荧光探针的原理和特征,尤其是遗传编码荧光蛋白探针的原理和应用,理解荧光探针在基础研究和疾病相关研究中的应用,并展望其在细胞及动物整体水平新的应用前景。
Redox state indicated by reactive oxygen species(ROS)level is a cellular physiological feature.Redox status is exactly controlled by conserved system.Its alterations consequently modulate various biological activities and play important roles in cells and organism under physiological and pathological conditions.Hence detection of redox state is deemed as an important technique both in basic and clinical researches.The present article reviews the principle and current applications of fluorescent probes or sensors for redox detection.With comparison of properties of various probes,especially genetic coding fluorescence probes,much understanding will be gained.And the review helps us to understand how to apply the redox probes on the biological and clinical researches and eventually look forward to new applications in the cell and whole animal level.
作者
刘奥
祁星
张颖超
徐天雪
易静
杨洁
LIU Ao;QI Xing;ZHANG Ying-chao;XU Tian-xue;YI Jing;YANG Jie(Department of Biochemistry and Moleculnr Cell Biology,Shanghai Jiao Tong Univers School of Medicine,Shanghai Key Laboratoryfor Tumor Microenvironment and Inflammation,Shanghai 200025,China)
出处
《上海交通大学学报(医学版)》
CAS
CSCD
北大核心
2018年第1期101-107,共7页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(31230037)
上海市自然科学基金(16ZR1418400)
