摘要
试验旨在研究季节对牛源E.coli O157∶H7分布的影响。在新疆地区A、B、C 3个牛场中,分别于春、夏、秋、冬4个季节采集牛肛拭子(399份)、粪便(68份)、水(29份)和饲料(43份)样本,先用EC肉汤增菌,再用山梨醇麦康凯培养基(SMAC)与4-甲基伞形酮-β-D葡萄糖醛酸苷(MUG)选择性培养,然后进行PCR鉴定和毒力基因检测。结果显示,从3个牛场539份样本中共检出E.coli O157∶H7菌株5株(0.93%,5/539),其中春季样本检出2株(1.44%,2/139),秋季样本检出1株(0.56%,1/180),冬季样本检出2株(1.38%,2/145),未能从夏季样本中检出目标菌;B牛场的菌株检自肛拭子样本(0.69%,1/145),C牛场的菌株分离于饲料(20.00%,3/15)和肛拭子(0.66%,1/152)样本,水中未分离到目标菌。牛源E.coli O157∶H7的分布具有明显的季节性,B牛场只在秋季的肛拭子样本中检出了1株E.coli O157∶H7,C牛场分别在春季和冬季各检出2株,春、冬季E.coli O157∶H7分离率高于夏、秋季。因此,在新疆特殊的气候特点和饲养模式下,防控牛源E.coli O157∶H7的传播时,要十分注意寒冷季节圈舍内卫生的管理,尤其要严防饲料被粪便污染。
To study the impact of season to the distribution of bovine E.coli O157∶H7,samples of anus swabs（399）,feces（68）,water（29）and feed（43）were collected in the spring,summer,autumn and winter from A,B and C farms of Xinjiang.After enrichment by EC broth,SMAC and MUG selective culture were then performed.Finally,PCR was used for identification and virulence gene detection of isolated strains.A total of 5 E.coli O157∶H7 strains were isolated from539 samples from three farms（0.93%,5/539）,2 of them were from spring（1.44%,2/139）,1 from autumn（0.56%,1/180）,2 from winter（1.38%,2/145）and no strains were isolated from summer samples.One strain were isolated from anus swab samples in farm B（0.69%,1/145）and one were isolated from anus swabs（0.66%,1/152）and three strains were isolated from feed samples in farm C（20.00%,3/15）,and no target strains were isolated from water samples.The distribution of bovine E.coli O157∶H7 had obvious seasonal characteristics.One E.coli O157∶H7 strain of farm B was isolated from autumn and four of farm C were from isolated spring（2 strain）and winter（2 strain）,and the isolation rate of E.coli O157∶H7 in spring and winter were higher than that in sum-mer and autumn.In conclusion,under the special climate characteristics and feeding mode in Xinjiang,to prevent and control the spreading of E.coli O157∶H7 of cattle,we must pay great attention on hygiene management of pens at cold season,specially avoiding the feed contaminated by feces.
出处
《中国畜牧兽医》
CAS
北大核心
2018年第1期212-218,共7页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31560485)
自治区重点研发计划项目(2017B01001-2)
新疆维吾尔自治区自然科学基金资助项目(2014211A028)
