摘要
目的研究侧脑室内注射瘦素对2型糖尿病大鼠阿黑皮素原(POMC)、神经肽Agouti相关蛋白(AGRP)及空腹血糖的影响。方法选取雄性8周龄SD 2型糖尿病大鼠80只,分为侧脑室内注射瘦素组和侧脑室内注射赋形剂(vehicle)对照组,各40岁。对瘦素注射组大鼠进行侧脑室5μL/kg(空腹瘦素水平15ng/mL)侧脑室瘦素注射,分别以等剂量对对照组进行vehicle侧脑室注射。48h后进行大鼠空腹血糖监测并使用ELISA分析检测试剂盒对两组大鼠血清标本进行POMC及AGRP滴度测定。结果注射瘦素组大鼠空腹血糖水平显著低于对照组(P<0.05),注射瘦素组大鼠血清POMC较对照组表达显著增加(P<0.05),AGRP表达显著降低(P<0.05)。结论侧脑室注射瘦素可显著降低2型糖尿病大鼠空腹血糖水平,增加POMC表达,降低AGRP表达。
Objective To study the effect of intracerebroventricular injection of leptin on proopiomelanocortin（POMC） and neuropeptide Agouti related protejn（AGRP） and fasting blood glucose （FBG） in rats with type 2 diabetes mellitus （T2DM）. Methods Eighty male 8-week-age SD rats with T2DM were selected and divided into two groups：intracerebroventricular injection of leptin group（n= 40）and intracerebroventricular injection of vehicle control group（n = 40）. Intracerebroventricular injection of 5 μL/kg（fasting leptin level 15 ng/mL） leptin was performed in the leptin injection group, and the control group was injected with same amount of vehicle instead. After 48 h, the FBG level was determined, and the levels of POMC and AGRP were detected by the titer method. Results The level of FBG in the leptin injection group was significantly lower than that in the control group（P〈0.05）, the expression of serum POMC in the leptin injection group was significantly higher than that in control group（P〈0.05）, The expression of AGRP in the leptin injection group was significantly decreased than control group（P〈0.05）. Conclusion intrac-erebroventricular injection of leptin can significantly reduce the FBG level in rats with T2DM,increases POMC expression and decreases AGRP associated protein expression.
出处
《重庆医学》
CAS
2018年第3期337-339,共3页
Chongqing medicine
基金
天津市北辰区科学技术委员会(BC2014-20)
关键词
糖尿病
2型
侧脑室
瘦素
黑皮质素类
神经肽类
diabetes mellitus, type 2
lateral ventricles
leptinl melanocortins
neuropeptides
