摘要
富硒酵母在医学保健和制药产业中有着重要的应用,高富硒酵母是富硒酵母产品开发的基础。以新疆富硒土壤为样品来源,通过马丁培养基的初筛和富硒能力的复筛,筛选到一株富硒量相对较高的黏红酵母菌株Rhodotorula glutinis X-20。优化了菌株的富硒培养条件,结果显示,利用2%甘油为碳源可显著提高酵母中硒的累积,培养12 h时添加50μg/m L的亚硒酸钠能够保证酵母较高的硒累积量和生物量,添加0.4 mmol/L的磷酸盐可以有效地促进硒的转运和累积,最终使Rhodotorula glutinis X-20富硒量达到5 009μg/g。
Selenium yeast is important for health and pharmaceutical industry,and high selenium-enriched yeast is the basis for the development of selenium-enriched yeast products. In this study,Rhodotorula glutinis X-20 with relatively high amount of enriched selenium was screened from selenium-enriched soil of Xinjiang via preliminary screening with Martin medium and re-screening of selenium-enriching capacity. The optimal culture conditions were determined through single experimental factors. The selenium content enriched by the yeast was significantly improved by using 2% glycerol as carbon source. Supplying sodium selenite at 12 h of cultivation ensured the higher selenium content and biomass of the yeast. Addition of 0.4 mM phosphate efficiently increased the transport and accumulation of selenium,and ultimately the enriched selenium content by strain X-20 reached 5009 μg/g.
出处
《生物技术通报》
CAS
CSCD
北大核心
2017年第12期132-137,共6页
Biotechnology Bulletin
基金
新疆兵团科技攻关计划(2016AB009)
关键词
富硒土壤
黏红酵母
筛选
优化培养
selenium-enriched soil
Rhodotorula glutinis
screen
optimized culture
