摘要
血尿素氮测定是肾功能检查和急诊患者最常用和最重要的项目之一.作者研制成功的两种干试剂法。操作简单,不必使用大型的生化分析仪器,也无需标本离心等步骤,只需指(耳)或静脉全血即可在4min内获得结果,能满足急诊患者肾功能状况的诊断和鉴别诊断的要求,适合于各种级别医院的急诊应用. 尿素氮试纸条制备方法:将脲酶、指示剂、缓冲剂和其它助剂固相在1张滤纸上。
This paper introduces a dry chemical method for BUN determination and a procedure for the preparation of the BUN Test Strip. Urease, indicator, buffer, nonreactive stabilizers and fillers are immobilized on a piece of special paper, and then a thin film is laid on top to protect against interference from endogenous blood materials. After the paper is dried, it is cut into small strips and ready to use. When the sample is loaded on the strip, the enzymatic reaction can be carried out in one minute, and with a color plate, one can obtain semiquantitative BUN concentration. One-day and day-to-day precision variations (CV%) were 2.6%-5.0% and 5.9%-7.1%, respectively. There was a good correlation between our BUN Strip and the Ames BUN Strip. r=0,98, y = 0,96x -2.4 (x=Ames BUN Strip).
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1991年第6期447-449,共3页
Acta Academiae Medicinae Sinicae
