摘要
本实验观察模拟海拔5000m连续缺氧20d和模拟海拔8000m连续缺氧4d对大鼠肺动脉内皮依赖性和非内皮依赖性舒张反应的影响。结果表明慢性缺氧显著抑制肺内和肺外动脉对乙酰胆碱和硝普纳舒张反应的敏感性和反应性。其机理很可能是由于缺氧抑制鸟苷酸环化酶,从而减低肺动脉对乙酰胆碱和硝普钠舒张反应的敏感性和反应性。
This experiment was designed to investigate whether chronic hypoxia could reduce pulmonary artery relaxation induced by acetylcholine and sodium nitrop-russide (endothelium-dependent and endothelium-independent vasodilator, respectively). Male Wistar rats were divided into 3 groups. Control, kept in air; CH4, in hypobaric chamber (8000 m above sea level) for 4 days; CH20. in hypobaric chamber (5000 m above sea level) for 20 days. All the hypoxic rats developed pulmonary hypertension. Rings of extra- (EPPA) or intra- (IPPA) pulmonary artery were suspended in an organ bath containing oxygenated Kreb's solution at 37Z℃ for relaxation/inhibition studies.The results showed that chronic hypoxia, CH4 and CH20, inhibited the relaxation response of both IPPA and EPPA to both acetylcholine and sodium nitroprusside. It is suggested that chronic hypoxia might attenuate the sensitivity and reactivity of the pulmonary artery to both acetylcholine and sodium nitroprusside through the inhibition of guanylcyclase activity in smooth muscle.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1991年第6期412-416,共5页
Acta Academiae Medicinae Sinicae
关键词
缺氧
肺动脉
乙酰胆碱
硝普钠
hypoxia pulmonary artery acetylcholine sodium nitroprusside
