摘要
目的评价紫斑牡丹花粉的不同浓度乙醇提取物的抗氧化能力。方法通过测定紫斑牡丹花粉不同浓度乙醇提取物的3种抗氧化能力(铜离子还原能力、DPPH自由基清除能力、ABTS自由基清除能力),对紫斑牡丹花粉不同浓度乙醇提取物的抗氧化性能进行评价。结果铜离子还原能力法测得紫斑牡丹花粉无水乙醇提取物、75%乙醇提取物、50%乙醇提取物、25%乙醇提取物和水提取物的抗氧化活性没食子酸量依次为26.00、28.33、28.90、14.98、9.24 mg/g;紫斑牡丹花粉不同浓度乙醇提取物的清除DPPH自由基和ABTS自由基能力均低于维生素C,其顺序依次为:50%乙醇提取物>75%乙醇提取物>无水乙醇提物>25%乙醇提取物>水提取物。结论紫斑牡丹花粉不同浓度乙醇提取物均具有较强的抗氧化能力,以50%、75%乙醇提物的抗氧化能力最强。
Objective To evaluate the antioxidant activity of different concentrations of alcohol extracts from Paeonia Rockii pollen. Methods The antioxidant activity of different concentrations of alcohol extracts from Paeonia Rockii pollen was evaluated by cupric reducing power method, DPPH radical scavenging activity method and ABTS radical scavenging activity method. Results The amounts of antioxidant activity of gallic acid of anhydrous ethanol extract, 75% alcohol extract, 50% alcohol extract, 25% alcohol extract, and water extract from Paeonia Rockii pollen were 26.00, 28.33, 28.90, 14.98, and 9.24 mg/g, respectively. The sequence of the ability of scavenging DPPH free radical and ABTS radical was Vc 〉 50% alcohol extract 〉 75% alcohol extract 〉 anhydrous ethanol extract 〉 25% alcohol extract 〉 water extract. Conclusion The different concentrations of alcohol extracts from Paeonia Rockii pollen has relatively strong antioxidant activity, especially for 50% alcohol extract and 75% alcohol extract.
出处
《中国中医药信息杂志》
CAS
CSCD
2018年第2期65-68,共4页
Chinese Journal of Information on Traditional Chinese Medicine
基金
兰州市科技计划项目(2016-3-17)
关键词
紫斑牡丹花粉
不同浓度乙醇提取物
抗氧化性能
铜离子还原能力法
DPPH自由基清除法
ABTS自由基清除法
Paeonia Rockii pollen
different concentrations of alcohol extracts
antioxidant activity
cupric reducing power method
DPPH radical scavenging activity method
ABTS radical scavenging activity method
