摘要
目的:探讨肝癌组织中Runx2表达量与癌细胞生长及细胞外基质降解的相关性。方法:选择在黄冈市中心医院进行肝癌根治术的原发性肝癌患者89例,留取术中肝癌组织及癌旁正常组织标本各89份,分别作为肝癌组、正常对照组。检测标本组织中Runx2、增殖相关基因、凋亡相关基因、MMPs等表达量,采用Pearson检验评估肝癌组织中Runx2mRNA表达量与细胞增殖、凋亡及细胞外基质分解的相关关系。结果:肝癌组标本中Runx2mRNA的表达量高于正常对照组标本中;肝癌组标本中增殖相关基因NFAT2mRNA的表达量低于正常对照组标本,DNMT3B、LAMP2、STC2、Versican、Bub1mRNA的表达量高于正常对照组标本;肝癌组标本中凋亡相关基因Survivin、Vimentin、Bcl-2、cIAP2mRNA的表达量高于正常对照组标本,Bax mRNA的表达量低于正常对照组标本;肝癌组标本中MMP-2、MMP-9、MMP-14mRNA的表达量均显著高于正常对照组标本中。Pearson检验发现,肝癌组织中Runx2mRNA表达量与细胞增殖活性、凋亡活性、MMPs表达量均直接相关。结论:肝癌组织中Runx2异常高表达,可直接参与癌细胞增殖活跃、凋亡异常等进程,具体机制与其促进细胞外基质降解相关。
Objective:To investigate the correlation of Runx2 expression with cancer cell growth and extracellular matrix degradation in liver cancer tissue.Methods:89 patients with primary liver cancer who received radical operation for liver cancer in Huanggang Central Hospital between January 2016 and August 2017 were selected,89 cases each of liver cancer tissue specimens and adjacent normal tissue specimens were kept during operation and enrolled as liver cancer group and normal control group respectively.The expression of Runx2,proliferation-related genes,apoptosis-related genes,MMPs and so on in the specimens were detected,and Pearson test was used to evaluate the correlation of Runx2 mRNA expression in liver cancer tissue with cell proliferation and apoptosis ans well as extracellular matrix degradation.Results:Runx2 mRNA expression in liver cancer group was higher than that in normal control group;proliferation-related gene NFAT2 mRNA expression in liver cancer group was lower than that in normal control group whereas DNMT3 B,LAMP2,STC2,Versican and Bub1 mRNA expression were higher than those in normal control group;apoptosis-related genes Survivin,Vimentin,Bcl-2 and cIAP2 mRNA expression in liver cancer group were higher than those in normal control group whereas Bax mRNA expression was lower than that in control group;MMP-2,MMP-9 and MMP-14 mRNA expression in liver cancer group were significantly higher than those in control group.Pearson test showed that the Runx2 mRNA expression in liver cancer tissue was directly correlated with cell proliferation activity,apoptosis activity and MMPs expression.Conclusion:There is abnormally high expression of Runx2 in liver cancer tissue,it can directly participate in the active cancer cell proliferation,abnormal apoptosis and other processes,and the specific mechanism is related to its effect on promoting extracellular matrix degradation.
出处
《海南医学院学报》
CAS
2018年第1期90-93,共4页
Journal of Hainan Medical University
基金
湖北省黄冈市科研基金项目(KJS0012)~~
