摘要
目的:研究臭氧联合透明质酸钠腔内注射对膝骨关节炎炎症介质、氧化应激分子的影响。方法:选择在本院诊断为中重度膝骨关节炎的患者并随机分为两组,试验组接受臭氧联合透明质酸钠腔内注射,对照组接受透明质酸钠腔内注射。治疗前及治疗后分别留取关节液标本,测定炎症及氧化应激信号分子、炎症介质、氧化应激分子的含量。结果:治疗后,两组患者关节液内p38MAPK、MEK-3/6、NFkB、TAK1、CXCL12、YKL40、IL1β、TNFα、PGE2、MDA、GRP78、CHOP的含量均显著低于治疗前,GPx的含量高于治疗前且试验组患者关节液中p38MAPK、MEK-3/6、NF-kB、TAK1、CXCL12、YKL40、IL1β、TNFα、PGE2、MDA、GRP78、CHOP的含量均显著低于对照组,GPx的含量高于对照组。结论:臭氧联合透明质酸钠腔内注射能够抑制膝骨关节炎患者局部的炎症反应和氧化应激反应。
Objective:To study the effects of ozone combined with sodium hyaluronate intracavitary injection on inflammatory mediators and oxidative stress in knee osteoarthritis.Methods:Patients who were diagnosed with moderate to severe knee osteoarthritis in Tangshan Hongci Hospital between July 2014 and March 2017 were selected and randomly divided into two groups,experimental group accepted ozone combined with sodium hyaluronate intracavitary injection,and control group accepted intracavitary injection of sodium hyaluronate.The joint fluid samples were collected before and after treatment respectively to determine the contents of inflammatory and oxidative stress signal molecules,inflammatory mediators and oxidative stress molecules.Results:After treatment,p38 MAPK,MEK-3/6,NF-kB,TAK1,CXCL12,YKL40,IL1β,TNFα,PGE_2,MDA,GRP78 and CHOP levels in joint fluid of both groups of patients were significantly lower than those before treatment whereas GPx levels were higher than those before treatment,and p38 MAPK,MEK-3/6,NF-kB,TAK1,CXCL12,YKL40,IL1β,TNFα,PGE_2,MDA,GRP78 and CHOP levels in joint fluid of experimental group were significantly lower than those of control group whereas GPx level was higher than that of control group.Conclusion:Ozone combined with sodium hyaluronate intracavitary injection can inhibit the local inflammatory response and oxidative stress response in patients with knee osteoarthritis.
出处
《海南医学院学报》
CAS
2018年第1期41-44,共4页
Journal of Hainan Medical University
基金
唐山市科技局项目(15CT201)~~
关键词
膝骨关节炎
臭氧
炎症反应
氧化应激反应
信号通路
knee osteoarthritis
ozone
inflammatory response
oxidative stress response
signaling pathway
