摘要
用流式细胞分析和图象分析技术,测定照射和加温后HeLa S_3细胞周期及DNA含量的变化。结果表明照射后HeLa S_3细胞DNA含量增加,照射及加温后HeLa S_3细胞出现G_2+M期延搁现象,提示DNA含量增加是G_2+M期延搁的结果。G_2+M期显著延搁时,细胞克隆源性存活率亦明显降低,X线照射细胞存活低于1％,加温处理细胞存活低于2.65％,提示G_2+M延搁现象不仅是细胞周期受阻的结果,也是细胞严重杀伤的后果。本实验证实,流式细胞分析和图象分析技术是快速测定受照和加温细胞反应的有效手段。
The effects of irradiation and hyperthermia on the distribution in various phases and DNA content of HeLa S3 cells were analyzed by flow cytometry and an image analysis instrument.A marked increase in DNA content from 6.718 to 9.614(AU) in HeLa S3 cells after 6 Gy irradiation was seen to correspond with the changes in the distribution of various phases in G2 + M, from 22%-52%. Meanwhile, the surviving fraction of HeLa S3 cells after 6 Gy irradiation was less than 1%. However, after heating at 44℃ for 10 min,the amount of cells in G2+M% increased from 22.5% to 52.5% and the surviving fraction after hyperthermia was less than 2.65%.The changes in distribution of various phases after Ir-192 irradiation were similar to those seen after X-ray irradiation.The delay of G2 + M phase after treatment with 8 Gy plus heating at 44癈 for 7 min in HeLa S3 cells was similar to that seen in the case of treatment with 8 Gy alone.As the surviving fraction accompanying the G2 + M delay after irradiation plus heat treatment was very low, we suggest that the changes of distribution in various phases of HeLa S3 cells after treatment might be used as a rapid indicator of serious injury.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1991年第5期358-362,共5页
Acta Academiae Medicinae Sinicae
关键词
照射
加温
细胞周期
流式细胞分析
radiation effect hyperthermia cell cycle flow cytometry
