摘要
目的:建立高效液相色谱法测定消银软膏中水杨酸含量的方法。方法:色谱柱为Wonda Cract ODS-2柱(4.6 mm×150 mm,5μm);流动相为水-甲醇-冰醋酸溶液(V:V:V=50:50:1);流速为0.9 ml/min;柱温为25℃;进样量为20μl;检测波长为304 nm。结果:水杨酸质量浓度在15.49~77.41μg/ml范围内呈良好的线性关系,线性回归方程为Y=43.311 X+10.93(R^2=0.999 8,n=6);水杨酸的加样回收率为98.3%(RSD=0.6%,n=9)。结论:本方法操作简便,灵敏度高,专属性强,为消银软膏的质量控制提供依据。
OBJECTIVE： To establish the method of high performance liquid chromatography （HPLC） for determining the contents of salicylic acid in Xiaoyin ointment. METHODS： The column was WondaCract ODS-2 （4. 6 mm× 150 mm,5μm） ; the mobile phase was water-methanol-glacial acetic acid （ V： V： V =50 ： 50：1 ） ; the flow rate was 0. 9 ml/min, the column temperature was 25 ℃, the injection volume was 20 μl and the detection wavelength was set at 304 nm. RESULTS： The calibration curves of salicylic acid was in good linearity over the ranges of 15.49- 77.41μg/ml, the linear regression model was Y = 43. 311 X ＋ 10. 93 （ R2 = 0. 999 8, n = 6 ） ; and the average recoveries was 98.3% （RSD = 0. 6% ,n = 9）. CONCLUSIONS： This method is simple, and has high sensitivity and strong specificity, which can provide reference for the quality control of Xiaoyin ointment.
出处
《中国医院用药评价与分析》
2017年第12期1655-1657,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
