摘要
为了解南城淮山脱毒苗移栽驯化期的光合生理,本研究对其移栽驯化期的叶绿素含量以及其光合特性和叶绿素荧光参数进行测定和统计分析。结果表明,南城淮山脱毒苗在移栽驯化初期具有较高的叶绿素含量;随着移栽驯化时间延长,脱毒苗的叶绿素含量也显著提高;当移栽驯化时间达到10 d时,脱毒苗的叶绿素含量达到最高值;但当移栽驯化时间超过10 d时,脱毒苗的叶绿素含量则显著下降。脱毒苗在移栽驯化期间的叶绿素含量显著高于其带毒苗。脱毒苗移栽驯化期内的净光合速率(Pn)、气孔限制值(Ls)、水分利用效率(WUE)、瞬时羧化速率(CUE)、初始荧光(Fo)、暗适应下最大荧光(Fm)、暗适应下PSⅡ最大光化学效率(Fv/Fm)、PSⅡ潜在光化学效率(Fv/Fo)、光适应下PSⅡ实际光化学效率(ФPSⅡ)、光适应下PSⅡ最大光化学效率(Fv'/Fm')、光化学荧光猝灭系数(q P)先增后降;蒸腾速率(Tr)和气孔导度(Gs)显著下降;胞间CO2浓度(Ci)和非光化学猝灭系数(q N)先降后升。脱毒苗的Pn、Tr、Gs、Ls、WUE、CUE、Fo、Fm、Fv/Fm、Fv/Fo、ФPSⅡ、Fv'/Fm'、q P均显著高于带毒苗,而其Ci和q N显著低于带毒苗。因此,南城淮山脱毒苗老叶在移栽驯化期第10天具有较强的光合效率,随后其光合效率随着移栽驯化时间的延长而显著下降,并逐渐由新生叶的光合作用所代替。南城淮山脱毒苗的光合效率显著高于带毒苗,有利于积累更多的光合产物,从而实现增产。本实验结果为南城淮山脱毒苗的移栽驯化及其大田种植提供了理论基础。
In order to understand the photosynthetic physiology of virus-free plantlets of Dioseorea opposite Thunb. cv. Nancheng during transplanting and domestication, the chlorophyll content, the photosynthetic characteristics and chlorophyll fluorescence parameters during transplanting and domesticatio were tested and statistically analyzed. The results showed that virus-free plantlets of D. opposite Thunb. cv. Nancheng had high chlorophyll content at the early stage of transplanting and domestication. The chlorophyll content of virus-free plantlets significantly increased with the time of transplanting and domestication. When the transplanting and domestication time reached 10 d, the chlorophyll content of virus-free plantlets reached the highest value. But when the transplanting and domestication time was over 10 d, the chlorophyll content decreased significantly. The chlorophyll content of virus-free plantlets was significantly higher than that of its plantlets with viruses. During transplanting and domestication of vires-free plantlets, Pn, Ls, WUE, CUE, Fo, Fm, Fv/Frn, Fv/Fo, ФPS Ⅱ, Fv＇/Fm＇ and qP increased firstly and then decreased, Tr and Gs significantly decreased and Ci decreased firstly and then increased. Pn, Tr, Gs, Ls, WUE, CUE, Fo, Fm, Fv/Fm, Fv/Fo, ФPS Ⅱ, Fv＇/Fm＇ and qP of virus-free plantlets was significantly higher than those of plantlets with viruses, but Ci and qN was significantly lower than those of plantlets with viruses. The old leaves of virus-free plantlets of D. opposite Thunb. cv. Nancheng had higher photosynthetic efficiency at the 10th day of transplanting and domestication, but its photosynthetic efficiency decreased gradually with the extension of time and was replaced by the photosynthesis of new leaf. The photosynthetic efficiency of virus-free plantlets of D. opposite Thunb. cv. Nancheng was significantly higher than that of plantlets with viruses, which was more conducive to the accumula- tion of photosynthetic products and realize high production. These results provide a theoretical basis for transplant- ing and domestication of virus-free plantlets of D. opposite Thunb. cv. Nancheng and their planting in the field.
作者
洪森荣
Hong Senrong(College of Life Science, Shangrao Normal University, Shangrao, 334001)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第11期4823-4828,共6页
Genomics and Applied Biology
基金
江西省教育厅2014年度科学技术研究一般项目(项目批准号:GJJ14712)资助
关键词
南城淮山
脱毒苗
移栽驯化
叶绿素含量
光合特性
叶绿素荧光参数
Dioseorea opposite Thunb. cv. Nancheng, Virus-free plantlet, Transplanting and domestication, Chlorophyll content, Photosynthetic characteristics, Chlorophyll fluorescence parameters
