摘要
在低超声波功率250 W条件下,利用响应面法优化铁皮石斛多糖提取工艺。根据Box-Behnken设计原理对提取温度、液料比、超声波处理时间进行中心组合试验,结果表明最佳提取条件为提取温度41.74℃、液料比50.26∶1(m L/g)、超声波处理时间28.65 min,此条件下铁皮石斛多糖得率为25.39%。比文献报道传统热水浸提最佳条件下多糖得率提高了18%,提取时间为传统热水浸提的1/4,且超声波辅助法并没有造成多糖清除1,1-二苯基-2-三硝基苯肼自由基能力、清除羟自由基能力的降低。利用不同剂量铁皮石斛多糖喂食果蝇,结果表明中剂量组为最佳剂量组,雄果蝇超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活力提高了30%,丙二醛(malondialdehyde,MDA)含量降低了46%;雌果蝇CAT活力提高了60%,SOD活力提高了6%,MDA含量降低了20%,雌、雄果蝇的平均最高寿命提高了20%。
The ultrasonic-assisted extraction of polysaccharide from Dendrobium officinale at a 250 W low power was optimized by response surface methodology based on central combination design. Extraction temperature, solvent-to-solid ratio, and ultrasonic treatment time were considered as independent variables. The optimum extraction conditions were determined as follows： extraction temperature 41.74 ℃, solvent-to-solid ratio 50.26：1（m L/g）, and ultrasonic treatment time 28.65 min. Under these conditions, the maximum extraction yield of 25.39% was obtained,which was increased by 18%compared with the value obtained using conventional hot water extraction with a simultaneous reduction in extraction time of 75%. Moreover, the DPPH and hydroxyl radical scavenging activities of polysaccharide from D.officinale were not reduced during ultrasonic-assisted extraction.Drosophila melanogaster were fed with different doses of the extracted polysaccharide,and the results showed that the polysaccharide at moderate dose provided the best antioxidant activity as evidenced by a 30% increase in superoxide dismutase（SOD） and catalase（CAT） activity and a 46% decrease in malondialdehyde（MDA） content of male D.melanogaster,as well as an increase of 60% in CAT activity and 6% in SOD activ ity and a 20% decrease in MDA content of female D.melanogaster,extending the lifespan by an average of 20%.
作者
邱现创
赵宁
李晨
王景雪
QIU Xianchuang;ZHAO Ning;LI Chen;WANG Jingxue(School of Life Science, Shanxi University, Taiyuan 030006, Chin)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第2期273-280,共8页
Food Science
关键词
铁皮石斛
多糖
超声波
响应面优化
抗氧化
果蝇
Dendrobium offcinale
polysaccharide
ultrasonic
response surface analysis
antioxidant activity
Drosophila melanogaster
