摘要
目的:研究广西汉族人群MTHFR、MTRR基因多态性与深静脉血栓的关系,为临床防治提供参考。方法:选取90例广西汉族下肢深静脉血栓患者作为研究组,另选取同期接受健康体检的90例广西汉族正常人员作为对照组,通过等位基因序列的特异性引物相关聚合酶链反应多态性技术对两组人员的MTHFR、MTRR基因多态性进行分析,进而对两组人员的基因型、等位基因相关分布频率进行比较。结果:研究组患者的MTHFR基因677位点的CC、TT、CT基因型及等位基因C、T的分布频率分别是41.1%、32.2%、26.7%、54.4%、45.6%,与对照组的55.6%、18.9%、25.5%、70.6%、29.4%比较,差异有统计学意义(P<0.05);而研究组人员的MTRR基因66位点AA、GG、AG基因型及等位基因A、G分布频率分别是56.7%、18.9%、24.4%、68.9%、31.1%,与对照组的48.9%、14.4%、36.7%、66.7%、33.3%比较,差异无统计学意义(P>0.05)。结论:广西汉族人群的深静脉血栓形成可能与MTHFR基因677位点的TT基因多态性存在相关性,而MTRR基因多态性与深静脉血栓发生可能无相关性。
Objective: To study the relationship between MTHFR and MTRR gene polymorphism and deep vein thrombosis in Guangxi Han population,and to provide reference for clinical prevention and treatment.Methods: 90 cases of patients with deep vein thrombosis of the lower limbs of Guangxi were selected as the study group.In the same period,90 cases of normal Chinese people in Guangxi were selected as the control group.The MTHFR and MTRR gene polymorphisms of the two groups were analyzed by the specific primer-related polymerase chain reaction polymorphism(PCR),to compare the genotype and allele-related distribution frequency data of the two groups of researchers.Results: The frequency of CC,TT,CT genotype and allele C and T of the MTHFR gene in the study group were 41.1%,32.2%,26.7%,54.4% and 45.6%,respectively,compared with 55.6%,18.9%,25.5%,70.6% and 29.4%of the control group(P < 0.05).The frequencies of AA,GG,AG genotype and allele A and G were 56.7%,18.9%,24.4%,68.9%and 31.1%,respectively,compared with 48.9%,14.4%,36.7%,66.7% and 33.3% of the control group(P > 0.05).Conclusion:The deep vein thrombosis of the Han population in Guangxi may be related to the TT gene polymorphism of the 677 locus of the MTHFR gene,and the MTRR gene polymorphism may not correlate with the occurrence of deep vein thrombosis.
出处
《川北医学院学报》
CAS
2017年第6期812-815,共4页
Journal of North Sichuan Medical College
基金
广西省桂林市科学研究与技术开发计划项目(20150206-1-9)
