摘要
应用基因特异性探针,通过Southern印迹杂交技术,分析五种有代表性的人肝癌细胞系基因组DNA中癌基因hst-1、int-2和c-sea等的结构。在所分析的肝癌细胞系基因组DNA中,未发现上述癌基因发生结构重排和扩增现象。这一结果对进一步研究人肝癌细胞中染色体畸变的分子基础有一定的参考价值。
Cytogenetic analysis has shown that 11q13, 8q24 and 11p15 are involvedin chromosome rearrangements found in human hepatoma cell lines. The oncogenesat 11q13 (hst-1, int-1 and c-sea), at 8q24 (c-myc) and at 11p15 (c-H-ras-1)may be implicated in human hepatocarcinogenesis. We have investigated thegenomic organization of the hst-1, int-2, c-sea, c-myc and c-H-ras-1 genesin DNA from 5 human hepatoma cell lines (PLC/PRF/5, Hep3B, SMMC-7721,BEL-7402 and Huh 7-4) with standard Southern technique. The results revealedno evidence for gross rearrangement and amplification of the genes analyzed.
出处
《中国医科大学学报》
CAS
CSCD
1991年第5期347-352,共6页
Journal of China Medical University
基金
国家自然科学基金
关键词
肝肿瘤
癌基因
染色体畸变
hepatoma
oncogene
chromosome rearrangement
