摘要
目的研究华南地区HCV基因型组成情况，了解HCV在华南地区的传播现状和推断其传播趋势。方法应用PCR荧光探针法或直接测序法对近两年华南地区的3 524份样本的HCV基因亚型进行检测和分析；对于PCR荧光探针法不能确定分型的标本，进一步采用套式PCR测序后进行系统发育树分析。结果3 524例样本中广东省2 922例，福建省78例，海南省152例和广西壮族自治区372例。1b亚型为最主要的基因亚型（1 808/3 524，51.3%），其次为6a（925/3 524，26.2%）、2a（298/3 524，8.46%）、3a（246/3 524，6.98%）、3b（200/3 524，5.68%）和1a（27/3 524，0.77%）；此外发现6e亚型1例、6q亚型1例、6r亚型1例、6w亚型3例、6xa亚型2例、6n亚型2例、基因型6＂特殊病毒株＂1例。广东省的21个市县中，大部分地区主要基因型为1b，其次为6a；部分地区主要基因型为6a，其次为1b；所有地区未见4型、5型、7型。结论近两年1b和6a仍是广东省、广西壮族自治区和海南省最主要的两个流行基因型；在广东省的部分地区6a已经取代1b成为最主要基因型；广西壮族自治区、福建省近两年HCV基因型分布上变化不大。
Objective To investigate the current distribution of hepatitis C virus （HCV） genotype in Southern China and to understand the HCV transmission and to infer its transmitting trend.MethodsThe HCV gene subtypes of 3 524 specimens from Southern China were detected and analyzed by polyonerase chain reaction （PCR）-fluorescence probe method or sequencing. The regular nested PCR and sequencing were used for the phylogenetic tree analysis when the fluorescence PCR inefficiently identifying virus isolates.ResultsAmong 3 524 specimens, there were 2 922 cases from Guangdong, 78 cases from Fujian, 152 cases from Hainan and 372 cases from Guangxi. Genotype 1b comprised the majority （1 808/3 524, 51.3%）, followed by genotype 6a （925/3 524, 26.2%）, 2a （298/3 524, 8.46%）, 3a （246/3 524, 6.98%）, 3b （200/3 524, 5.68%） and 1a （27/3 524, 0.77%）. In addition, 1 case was genotype 6e, 1 case was genotype 6q, 1 case was genotype 6r, 3 case were genotype 6w, 2 case were genotype 6xa, 2 case were genotype 6n, and 1 case was genotype 6 with unclassified subtype. The genotype 1b accounted for the majority in most areas of 21 cities and counties in Guangdong Province, followed by genotype 6a. But in some areas, the major genotype was genotype 6a, followed by 1b. Genotype 4, genotype 5 and genotype 7 were not found in this study.ConclusionsIn the past two years, genotype 1b and 6a are still the epidemic genotypes in Guangdong, Guangxi and Hainan provinces. However, genotype 6a has replaced 1b as the dominant one in some areas in Guangdong Province. The distributions of HCV genotypes do not change significantly in Guangxi and Fujian provinces.
出处
《中华传染病杂志》
CSCD
北大核心
2017年第10期605-611,共7页
Chinese Journal of Infectious Diseases
基金
海南省重点研发项目（ZDYF2017130）
