摘要
建立了高效液相色谱手性流动相添加剂法拆分辛弗林对映体的方法。选用C18色谱柱,以0.5 g/L羧甲基-β-环糊精(CM-β-CD)为添加剂,流动相为混合液(V(乙腈)∶V(甲醇)∶V(水)=70∶15∶15),紫外检测波长为202 nm,流量为0.2m L/min,pH 4.20。在此条件下,辛弗林对映体分离度为1.47。
The enantiomeric separation of Synephrine by HPLC with chiral mobile phase additive was established.The C18 column was used as stationary phase,using the 0. 5 g/L carboxymethyl-β-cyclodextrin（CM-β-CD） as chiral mobile phase additive,and a mixture of acetonitrile-methanol-water（mixed in the ratio of 70 ∶ 15 ∶ 15 by volume） was used as mobile phase,the UV detection wavelength was set at 202 nm,at a flow-rate of 0. 2 m L/min,pH 4. 20.Under the optimized conditions,the resolution of Synephrine enantiomer was 1. 47.
作者
翟明翚
于浩
刘呈利
孟培
苏立强
ZHAI Ming-hui;YU Hao;LIU Cheng-li;MENG Pei;SU Li-qiang(College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China)
出处
《化学试剂》
北大核心
2017年第12期1344-1346,1360,共4页
Chemical Reagents
基金
黑龙江省教育厅资助项目(12531757)
