摘要
目的优选胡柚幼果中柚皮苷与新橙皮苷的提取工艺。方法在单因素试验的基础上,对乙醇浓度、料液比、提取时间3个因素进行响应面Box-Behnken中心组合设计优化,得到最佳提取工艺。结果最佳提取工艺条件为乙醇浓度60%、料液比1∶10、提取时间1.8 h、回流提取2次,在此条件下柚皮苷与新橙皮苷的总得率为23.44%。结论本提取工艺方法简单、合理、稳定,为工业化生产提供了一定的依据。
OBJECTIVE To optimize the extraction process of naringin and neohesperidin from Huyou fruitlet. METHODS Based on the single factor experiment, Box-Benhnken three factors（ethanol concentration, solid-liquid ratio and extraction time） experiment by response surface design was carried out. RESULTS The optimum conditions was as follows： solid to liquid ratio 1∶10, 60% ethanol, extraction time 1.8 h and extraction two times. Under these conditions, the total yield of naringin and neohesperidin from huyou fruitlet was 23.44%. CONCLUSION The method is simple, reasonable, feasible and provide a experiment basis for its industrial production.
出处
《中国现代应用药学》
CAS
CSCD
2017年第11期1546-1551,共6页
Chinese Journal of Modern Applied Pharmacy
基金
湖南省高校科技创新团队支持计划资助(2012-318)
永州市科技计划资助项目(2016-8)
关键词
响应面分析法
胡柚幼果
柚皮苷
新橙皮苷
response surface methodology
Huyou fruitlet
naringin
neohesperidin
