摘要
目的探讨黄芪对实验性自身免疫性神经炎(EAN)的治疗作用。方法用周围髓鞘蛋白抗原180-199肽段(P0180-199)免疫Lewis大鼠,建立EAN模型,用黄芪水煎液灌胃治疗,观察其发病情况,HE染色检测坐骨神经炎性细胞浸润情况,ELISA检测干扰素γ(IFN-γ)、白介素17(IL-17)以及抗P0180-199抗体数目。结果黄芪减轻EAN临床症状,减少坐骨神经炎性细胞浸润,减少IFN-γ、IL-17以及抗P0180-199抗体的产生。结论黄芪具有EAN治疗潜能,为格林巴利综合征(GBS)的临床治疗提供新的策略。
Objective , To explore the effect and mechanism of astragalus membranaceus （AM） on experimental autoimmune neuritis （EAN）.Methods AM was administered intragastrically daily to EAN rats immu- nized with P0 peptide 180- 199.The clinical signs of rats were closely observed. HE staining was used to detect the inflammatory cell infiltration of sciatic nerve.IFN- γ, IL - 17 and anti- P0 peptide 180 - 199 antibod- ies were detected by ELISA.Results Treatment of AM resulted in amelioration of EAN,reduction of the inflammatory cell infiltration of sciatic nerve, and down- regulation of IFN - γ, IL- 17 and anti- P0 peptide 180- 199 antibodies.Conclusion AM： may he a potential therapeutic agent for EAN,and it provides a new therapeutic strategy for Guillain - BarrE syndrome.
出处
《中国煤炭工业医学杂志》
2017年第11期1354-1357,共4页
Chinese Journal of Coal Industry Medicine
基金
国家自然科学基金项目(编号:81671631)
