摘要
为了探索超声波处理在植物转化中的功效及潜在发展能力,从超声波辅助植物转化的原理入手,归纳了方法的验证、超声波处理的受体材料和超声波处理对外源DNA的影响;总结了前人在超声波辅助植物转化方面的研究进展,进而分析了方法自身的优缺点及存在问题。指出超声波辅助植物转化方法虽然处于初级发展阶段,但超声波辅助的植物转化仍然是一个操作简单、省时省力、适用范围广的植物转化方法,文中还提出了存在的问题且给出了相应的解决对策;通过分析认为超声波辅助的植物转化方法具有广阔的发展前景。
To evaluate the efficiency and potential development capacity of ultrasonic treatment for planttransformation, we started with the principle of ultrasonic-assisted plant transformation, summarized thevalidation of method, the receptor materials of ultrasonic treatment and the effects of ultrasonic treatment onexogenous DNA. Then, the research progresses in ultrasonic-assisted plant transformation were reviewed, andthe advantages as well as disadvantages and existing problems of the method were discussed. Our resultsindicated that although the development of ultrasonic-assisted plant transformation was still in the primarystage, it demonstrated that the method was a simple, time-saving, labour-saving and widely applied planttransformation approach. Moreover, we proposed respective solution to the existing problems. Taken together,we consider that ultrasonic-assisted plant transformation method has broad development prospect.
作者
王建军
冯莉赟
申虎飞
刘佼
Wang Jianjun;Feng Liyun;Shen Hufei;Liu Jiao(Xixian Agricultural Experiment Station, Shanxi Academy of Agricultural Sciences, Xixian 041399, Shanxi, China)
出处
《农学学报》
2017年第11期6-13,共8页
Journal of Agriculture
基金
国家转基因重大专项"抗病虫
抗除草剂转基因玉米新品种选育"(2016ZX08003001-002-003)
山西省科技攻关项目"多抗耐密型玉米新种质创制及新品种选育"(20140311002-1)
关键词
超声波
辅助
植物
转化方法
Ultrasonic
Assistance
Plant
Transformation Method
