摘要
目的 建立一种小型猪全层皮肤缺损创面模型 ,并用此模型研究碱性成纤维细胞生长因子 (bFGF)对创面愈合的作用。方法 小型猪背部剃毛 ,用特制致伤器 (直径 1 8mm打孔器 )在背部脊柱两侧垂直于皮肤向下钻至深筋膜 ,剪去已钻掉的皮肤 ,形成全层皮肤缺损创面。将创面分为bFGF治疗和空白对照两组 ,测定伤后不同时间创面面积、伤腔容积及进行创面组织病理学检查。结果 伤后 3、7dbFGF治疗组创面面积缩小到 (1 .81± 0 .1 5)cm2 、(0 .51± 0 .1 0 )cm2 ,明显小于空白对照组的 (2 .0 0± 0 .2 1 )cm2 、(0 .61± 0 .1 1 )cm2 (P <0 .0 5) ;伤腔容积缩小到 (0 .49± 0 .0 7)ml、(0 .0 2± 0 .0 4 )ml,也小于空白对照组的 (0 .61± 0 .1 7)ml、(0 .1 4± 0 .1 6)ml(P <0 .0 5)。伤后1 4d组织病理学检查可见bFGF组大部分创面上皮化 ,而空白对照组较多的创面未愈合。结论 经特制致伤器制备的小型猪全层皮肤缺损创面模型简便、快速 ,创面之间具有较好的均衡性 ,是研究外用药物促进创面修复作用的较理想模型之一 。
Objective To establish an experimental model of full thickness skin defect in minipigs and to investigate the effects of basic fibroblast growth factor (bFGF) on wound healing.Motheds After the skin on back of the minipigs was shaved, the cut skin in each side of spinal column was removed with a specific traumatoqenic apparatus (18?mm in diameter) to achieve a full thickness skin defect wound. The wounds were randomly divided into two groups (n=16): the wounds treated with bFGF served as the experimental group, while those not treated with bFGF as the control group. At the 3rd, 7th and 14th day after injury, the area of the wounds and wound volume were measured and the histological examination was performed to evaluate the wounds healing.Results Three and 7 days after injury, in the bFGF treated group, the area of wounds was decreased to (1.81±0.15)?cm 2 and (0.51±0.10)?cm 2 respectively, significantly less than in the blank control group (2.00±0.21)?cm 2 and (0.61±0.11)?cm 2 respectively) (P<0.05);The wound volume in the bFGF treated group was significantly decreased to (0.49±0.07)?ml and (0.02±0.04)?ml respectively, significantly smaller than in the non treated group (0.61±0.17)?ml and (0.14±0.16)?ml respectively) (P<0.05). Histological examination revealed that most of the wounds in the experimental group were re epithelialization at the 14th day, but in control group most of the wounds were not healed on day 14 after operation.Conclusion The wound model of full thickness skin defect in minipig holds good to study the effects of medicine for external use on wound healing. bFGF can promote the wound healing.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2002年第5期466-467,共2页
Chinese Journal of Experimental Surgery
