摘要
目的 探讨联合应用生长激素和谷氨酰胺防止小肠粘膜萎缩的分子学机制。方法 选用 40只手术成功的SD短肠大鼠 ,随机均分为 4组 ,分别给予常规全肠外营养 (TPN组 )、附加谷氨酰胺 (TPN +Gln组 )、附加生长激素 (TPN +GH组 )及附加谷氨酰胺和生长激素全肠外营养(TPN +GH +Gln组 ) ,持续 6d。另取 8只正常大鼠模拟手术后第 1天处死 ,作为基础对照组 (Con trol组 )。应用逆转录 聚合酶链式反应 (RT PCR)技术检测残留小肠粘膜上皮细胞c fos和c junmRNA的表达。结果 小肠粘膜上皮细胞c fos和c junmRNA表达值中 ,TPN组 (0 .1 1± 0 .0 7、0 .2 9± 0 .1 0 )明显低于对照组 (0 .48± 0 .1 5、0 .57± 0 .2 2 ,P <0 .0 5) ;TPN +Gln和TPN +GH组 (分别为 0 .32± 0 .1 5和 0 .48± 0 .2 1 ,0 .36± 0 .1 3和 0 .46± 0 .1 7) ,较TPN组显著增高 (P <0 .0 5) ;TPN +GH +Gln组 (0 .35± 0 .1 6、0 .61± 0 .1 3)明显高于TPN组 (P <0 .0 5) ,与对照组差异无显著性。结论 生长激素或谷氨酰胺单独应用均可通过上调小肠粘膜上皮细胞c fos和c jun基因的表达 ,促进细胞分裂增殖 ,从而防止小肠粘膜萎缩的发生 。
Objective To explore the molecular mechanism of combined treatment with growth hormone and glutamine preventing intestinal mucosal atrophy.Methods Forty Sprague Dawley rats with small bowel syndrome were randomized to four groups according to 2×2 factorial experiment and were treated with standard total parenteral nutrition (TPN group,n=10), glutamine enriched TPN (TPN+Gln group,n=10), growth hormone enriched TPN (TPN+GH group,n=10) or glutamine and growth hormone enriched TPN (TPN+Gln+GH group,n=10) respectively for 6 days. Eight normal sham operated rats served as baseline controls (control group,n=8). The expression of c fos and c jun mRNA in intestinal mucosa was detected by using RT PCR technique.Results The expression levels of c fos and c jun mRNA in the TPN group were 0.11±0.07 and 0.29±0.10, which were significantly decreased as compared with 0.48± 0.15 and 0.57±0.22 in the control group (P<0.05). The expression levels of c fos and c jun mRNA in the TPN+Gln or TPN+GH group were 0.32±0.15 and 0.48± 0.21 or 0.36±0.13 and 0.46±0.17, which were significantly increased as compared with thos in the TPN group (P<0.05); The expression levels of c fos and c jun mRNA in the TPN+Gln+GH group were 0.35±0.16 and 0.61±0.13, which were significantly increased as compared with those in the TPN group (P<0.05).Conclusion Growth hormone or glutamine treatment prevents intestinal mucosal atrophy possibly by up regulating expression of c fos and c jun in intestinal mucosa to promote intestinal cell proliferation. Moreover combined use of these two gut trophic factors can produce synergistic effects.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2002年第5期431-433,共3页
Chinese Journal of Experimental Surgery
基金
国家教委博士点基金资助项目 ( 9736 )
上海市卫生局科技发展基金资助项目 ( 974 2 9)
