摘要
干旱是最重要的环境胁迫,香蕉MaASR1基因在植物响应逆境胁迫时发挥着重要作用,为了深入研究MaASR1基因的过表达使拟南芥抗旱的分子机制,利用全基因组表达芯片来广谱的筛选MaASR1基因转入后自然条件下及干旱处理条件下差异基因的表达情况。对基因芯片的结果进行了详细的生物信息学分析及相关基因的RT-PCR验证,结果表明MaASR1基因异源表达的拟南芥株系在自然生长条件下共有747个差异基因,其中上调基因559个,下调基因188个;在干旱胁迫条件下共得到653个差异基因,其中上调基因256个,下调基因397个;MaASR1基因的转入可以通过影响激素、光合作用、锌指蛋白及不依赖ABA途径的DREB2A等相关基因的表达来提高拟南芥的抗旱性。为解析MaASR1基因作为转录因子提高植物抗旱能力的分子机制奠定基础。
Drought is the most important environmental stress. MaASR1 gene of banana plays an important role in plant responding to stress. In order to further study the molecular mechanism of drought resistance for over expressing MaASR1 gene in Arabidopsis thaliana. DNA mieroarray was used to broad-spectrum screening the differentially expressed genes under natural and drought treatment in wild-type Arabidopsis thaliana and transgenic lines. The results of the DNA microarray were analyzed by bioinformatics and RT-PCR verification of the related genes. The results showed that when the wild-type Arabidopsis thaliana and transgenic lines were all without any treatment, there was a total of 747 differentially expressed genes, including 559 up-regulated genes and 188 down-regulated genes. And when the wild-type Arabidopsis thaliana and transgenic lines were all droughttreated, there was a total of 653 differentially expressed genes, including 256 up-regulated genes and 397 downregulated genes. MaASR1 gene can increase the drought resistance of Arabidopsis thaliana by affecting the expression of hormone, photosynthesis, zinc finger protein and DREB2A which involved in the ABA-independent pathway. And to lay the foundation for the molecular mechanism of MaASR1 gene as a transcription factor to improve plant drought resistance.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2017年第11期59-73,共15页
China Biotechnology
基金
国家香蕉产业技术体系品种改良(CARS-31)
转基因生物新品种培育国家科技重大专项(2016ZX08012005-007)
海南省重大专项(HNGDpz201502)资助项目
