摘要
以伽师瓜为材料,研究贮藏期间果皮与果肉对病原菌抵抗能力的差异。分别采用3 mm(果皮)、5 mm(果皮果肉交界)、10 mm(果肉)深度接种链格孢(Alternaria alternata)后,于7℃贮藏。定期检测果皮与果肉病斑直径、总酚和黄酮含量及多酚氧化酶(PPO)、过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性。结果表明:贮藏期间,不同接种深度的果皮病斑直径显著小于果肉(p<0.05),果皮的抗病能力大于果肉;3、5、10 mm接种组果皮与果肉的抗菌物质含量和活性氧代谢相关酶活性显著高于对照(p<0.05),其中3 mm接种组果实具有较高的抗性;甜瓜果皮与果肉协同抵御链格孢的侵染,果皮发挥更大的抗病作用。
Take Jiashi Melon as the experimental materials, this study was aimed to study the resistance difference of pericarp and sarcocarp to pathogenic bacteria during storage.muskmelon was inoculated with A.alternata at different depths were 3 mm （pericarp） ,5 mm（pericarp and sarcocarp at the junction）and 10 mm（sarcocarp）, and stored at 7℃ cold storage.Regular inspection of the Lesion diameter of pericarp and sarcocarp, the contents of total phenols and flavonoids in antibacterial substances, and the activities of polyphenol oxidase （ PPO ）, peroxidase （ POD ）, superoxide dismutase （ SOD ） and catalase （CAT） activity.The results revealed that pericarp lesion diameter was significantly smaller than the sarcocarp（p 〈0.05 ）during the storage.The disease resistance of pericarp is greater than sarcocarp.The contents of antibacterial substances and reactive oxygen metabolism related enzymes in the 3,5 and 10 mm inoculated groups were significantly higher than those in the control group （p 〈 0.05 ）, the fruit of 3 mm inoculation group had higher resistance to disease, pericarp and sarcocarp cooperate to resist the infection of Alternaria alternata. DericarD exerts a greater resistance to disease.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第23期245-249,255,共6页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31460413)
关键词
厚皮甜瓜
链格孢
抗菌物质
活性氧代谢相关酶
muskmelon
Alternaria alternata
antibacterial substances
activity of enzymes related to reactive oxygen metabolism
