摘要
目的研究三七Panax notoginseng叶乙醇提取物的化学成分及抗氧化活性(ABTS和DPPH)。方法利用硅藻土、硅胶、MCI柱色谱、凝胶柱色谱、中压制备液相色谱和制备液相等色谱技术进行分离纯化,并应用质谱、核磁共振等波谱技术鉴定化合物结构。初步考察了这些化合物的抗氧化能力。结果从三七叶95%乙醇提取物的氯仿、丙酮-乙醇(4∶1)和丙酮-乙醇(1∶1)3个洗脱部位中分离鉴定了15个化合物,分别为falcarinol(1)、γ-tocopherol(2)、(5S)-3α-acetyl-2,3,5-trimethyl-7α-hydroxy-5-(4,8,12-trimethyltridecanyl)-1,3α,5,6,7,7α-hexahydro-4-oxainden-1-one(3)、picrionoside B(4)、linarionoside A(5)、viburnolide A(6)、槲皮素(7)、lilyn(8)、茵芋苷(9)、阿彼斯基姆素(10)、daphnin methylether(11)、20(S)-人参皂苷Rh2(12)、人参皂苷F2(13)、20(R)-人参皂苷Rh2(14)、异人参皂苷Rh3(15)。结论化合物3、6、9~11为首次从人参属植物中分离得到,化合物2、4为首次从该植物中分离得到。化合物2、4、7、8具有较强的抗氧化活性。
Objective To study the chemical constituents from the leaves of Panax notoginseng and their antioxidant activity(ABTS and DPPH).Methods The constituents were isolated and purified by celite,silica gel,MCI and Sephadex LH-20,reversed-phase MPLC chromatographic methods,then purified by preparative HPLC.Their structures were identified on the basis of mass spectrum and NMR spectrum.Anti-oxidant activity of these compounds was initially investigated.Results Fifteen compounds were isolated from the chloroform,acetone-ethanol(4:1)and acetone-ethanol(1:1)fractions of 95%ethanol extract of the leaves of P.notoginseng,and their structures were elucidated as follows:falcarinol(1),γ-tocopherol(2),(2 S,4αS,7αR)-7α-acetyl-3,4,4α,7α-tetrahydro-4α-hydroxy-2,6,7-trimethyl-2-(4,8,12-trimethyltridecyl)cyclopenta[β]pyran-5(2 H)-one(3),picrionoside B(4),linarionoside A(5),viburnolide A(6),quercetin(7),lilyn(8),skimmin(9),apiosylskimmin(10),daphnin methylether(11),20(S)-ginsenoside Rh2(12),ginsenoside F2(13),20(R)-ginsenoside Rh2(14),and isoginsenoside Rh3(15).Conclusion Compounds 3,6,and 9—11 are isolated from the plant of genus Panax for the first time,compounds 2 and 4 are isolated from this plant for the first time.Compounds 2,4,7,and 8 show certain anti-oxidant activity.
作者
黄积武
李创军
杨敬芝
马洁
张东明
HUANG Ji-wu;LI Chuang-jun;YANG Jing-zhi;MA Jie;ZHANG Dong-ming(State Key laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China)
出处
《中草药》
CAS
CSCD
北大核心
2017年第21期4381-4386,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金重点资助项目(81630094)
中国医学科学院医学与健康科技创新工程项目(2016-I2M-1-010)
