摘要
目的:探讨G蛋白耦联受体30(GPR30)mRNA和蛋白表达水平与子宫肌瘤的相关性。方法:选取2015年10月至2016年5月华中科技大学同济医学院附属同济医院妇产科收治的30例子宫肌瘤患者组织样本及子宫肌瘤旁正常平滑肌组织样本为研究对象。采用RT-PCR和western blotting检测并比较GPR30在子宫肌瘤组织及周围正常肌层组织中的mRNA和蛋白表达水平的差异。结果:与正常子宫肌层组织比较,子宫肌瘤组织GPR30 mRNA和蛋白表达均显著增高(均P<0.05);且子宫肌瘤组织中GPR30蛋白水平是正常子宫肌层组织中的3.9倍。与首发子宫肌瘤组织比较,复发子宫肌瘤组织中GPR30的mRNA和蛋白表达水平均显著增高(均P<0.05),且GPR30蛋白表达为首发子宫肌瘤的1.6倍;与直径<5 cm子宫肌瘤组织比较,直径≥5 cm子宫肌瘤组织中GPR30 mRNA和蛋白表达均显著增高(均P<0.05),且GPR30蛋白表达为直径<5 cm子宫肌瘤组织的1.6倍;与单发子宫肌瘤组织比较,多发子宫肌瘤组织中GPR30 mRNA和蛋白表达均显著增高(均P<0.05),且GPR30蛋白表达为单发子宫肌瘤的1.5倍。结论:GPR30与子宫肌瘤发生发展密切相关。
Objective： To evaluate the correlation of G protein-coupled receptor 30 （GPR30） and uterine fibroid. Methods： 30 patients with uterine fibroid treated in our hospital from Oct. 2015 to may 2016 were selected. The mRNA and protein expression levels of GPR30 in the tissues of uterine fibroid and normal myometrium were determined by western blotting and RT-PCR, respectively. Results： The mRNA and protein expressions of GPR30 in uterine fibroid tissues were significantly up-regulated, and the GPR30 protein level was 3.9 times higher than that in normal myometrium tissues （P〈 0. 05 ）. The patients with recurrent uterine fibroid had a significant increased in GPR30 mRNA and protein expression （P〈0. 05）, and the protein level of GPR30 was 1. 6 times higher than those without relapse. When the largest diameter of the fibroid ≥5 cm, the GPR30 mRNA and protein expressions were significantly elevated （P〈0.05）, and the protein level was 1.6 times higher than that of uterine fibroid with a diameter less than 5 cm. There was also significant difference in the mRNA and protein expression levels of GPR30 between solitary and multiple uterine leiomyomas （P〈0. 05）. Conclusion： GPR30 was closely related to the occurrence and progress of uterine fibroid.
出处
《广西医科大学学报》
CAS
2017年第11期1578-1581,共4页
Journal of Guangxi Medical University
