摘要
目的探讨砷对周围血淋巴细胞中及人肺腺癌细胞系A549细胞中同源异型盒D10(HOXD10)基因表达的影响。方法 (1)采用判断抽样方法,抽取某砒霜厂职业性接触砷的59名工人为接触组,以无职业性接触砷的17名当地居民为对照组。采用氢化物发生-冷肼捕集-原子吸收法检测2组人群尿中形态砷水平,采用实时荧光定量聚合酶链式反应(qRT-PCR)法检测周围血淋巴细胞中HOXD10的表达。(2)以浓度为0.0、0.1、0.5、1.0、2.0μmol/L的三氧化二砷(As_2O_3)处理A549细胞,采用比色法检测细胞存活率,采用qRT-PCR法检测HOXD10的表达。结果 (1)接触组人群尿中无机砷、一甲基砷酸、二甲基砷酸、总砷水平和周围血淋巴细胞中HOXD10 mRNA相对表达水平均高于对照组(P<0.05)。(2)As_2O_3可导致A549细胞存活率呈现剂量依赖性下降(P<0.01),HOXD10mRNA相对表达水平呈现剂量依赖性上调(P<0.01)。A549细胞存活率和HOXD10 mRNA相对表达水平呈负相关,相关系数为-0.777(P<0.01)。结论砷可导致职业接触人群周围血中HOXD10表达上调。As_2O_3可抑制A549细胞增殖,可能与上调HOXD10表达有关。
Objective To explore the effect of arsenic on the homeobox D10（ HOXD10） gene expression in peripheral blood lymphocytes and human lung adenocarcinoma cell A549. Methods ⅰ） A total of 59 workers exposed to arsenic from a arsenic factory were selected as the exposure group and 17 local people without arsenic exposure were chosen as controls by using judgment sampling method. Hydride generation-cold hydrazine trapping-atomic absorption spectrometry was used to detect arsenic levels in urine of these 2 groups. The expression of HOXD10 in peripheral blood lymphocytes was detected by real-time fluorescent quantitative polymerase chain reaction（ qRT-PCR）.ⅱ） The A549 cells were treated with arsenic trioxide（ As_2O_3） with concentration of 0. 0,0. 1,0. 5,1. 0 and 2. 0 μmol/L,and the survival rate of cells was examined by colorimetric assay. The expression of HOXD10 was detected by qRT-PCR. Results ⅰ） The levels of inorganic arsenic,methylarsonic acid,dimethyl arsenate,total arsenic in the urine,and the relative expression of HOXD10 mRNA in peripheral blood lymphocyte in exposure group were higher than that of the control group（ P〈0. 05）.ⅱ） As_2O_3 decreased the survival rate of A549 cells in a dose-dependent manner（ P〈0. 01） and lead to a dose-dependent increase of HOXD10 mRNA expression（ P〈0. 01）. A549 cell survival rate and relative expression of HOXD10 mRNA showed a negative correlation,the correlation coefficient was-0. 777（ P〈0. 01）. Conclusion Arsenic can up-regulate HOXD10 expression in the peripheral blood of occupational arsenic exposure individuals. As_2O_3 can inhibit the proliferation of A549 cells,which may be related to the up-regulation of HOXD10 expression.
出处
《中国职业医学》
CAS
北大核心
2017年第5期542-546,共5页
China Occupational Medicine
基金
国家自然科学基金(81460491)
云南省公共卫生与疾病防控协同创新项目(2016YNPHXT15)
