摘要
目的探讨钙网织蛋白(CRT)在类风湿关节炎(RA)早期诊断和疾病活动度评估中的作用及临床意义。方法收集45例早期活动性RA患者、35例骨性关节炎(OA)患者,以及30例正常对照者(HC)的血清标本。采用酶联免疫吸附法检测3组患者血清CRT水平,魏氏法检测红细胞沉降率(ESR),免疫散射比浊法检测C反应蛋白(CRP)的水平。采用Spearman对血清CRT水平与疾病活动度评分(DAS28)、ESR、CRP进行相关性分析。结果 RA组血清CRT水平[(6.7±2.5)μg/L]高于OA组[(3.9±0.9)μg/L]和HC组[(3.8±0.7)μg/L](P<0.05),OA组与HC组的血清CRT水平比较,差异无统计学意义(P>0.05);与OA组和HC组比较,RA组ESR、CRP水平升高(P<0.05);RA组血清CRT水平与DAS28评分、ESR及CRP呈正相关(rs=0.391、0.459和0.454,P=0.008、0.002和0.002)。结论早期活动性RA患者血清CRT水平增高,CRT与DAS28评分、ESR及CRP呈正相关,提示CRT可能成为早期诊断RA,以及辅助ESR、CRP评估RA疾病活动度的潜在血清学指标。
Objective To investigate the role of calreticulin(CRT) in the diagnosis and assessment of disease activity in the patients with early rheumatoid arthritis(RA). Methods Serum CRT level was measured by ELISA in 45 patients with early RA, 35 cases with osteoarthritis(OA) and 30 healthy controls(HC). ESR was measured by the Westergren method. Serum CRP level was measured by immunonephelometry. The correlations of CRT with Disease Activity Score for 28 joints(DAS28 score), ESR and CRP were assessed by Spearman's rank correlation coefficient. Results The serum concentration of CRT of the early RA patients[(6.7 ± 2.5) μg/L] was higher than that of the OA patients and HC [(3.9 ± 0.9) and(3.8 ± 0.7) μg/L, P < 0.05],while there was no significant difference between the OA and HC groups(P > 0.05). Compared with the OA and HC groups, the ESR and CRP levels were elevated in the early RA group(P < 0.05). Serum CRT concentration in the early RA patients was positively correlated with DAS28 score, ESR and CRP(rs=0.391,0.459 and 0.454; P = 0.008, 0.002 and 0.002). Conclusions Together with ESR and CRP, CRT may be used as a potential biomarker for early diagnosis of RA and for assessment of disease activity.
出处
《中国现代医学杂志》
CAS
北大核心
2017年第26期52-55,共4页
China Journal of Modern Medicine
基金
河北省科技计划项目(No:152777157)
