摘要
目的评估持续高频正弦波及方波频闪光对豚鼠眼球正视化及眼球发育的影响。方法 30只2周龄豚鼠分为20 Hz方波、20 Hz正弦波频闪组及无频闪对照组共3组(n=10),光照强度统一为500 lx,每2周测量豚鼠眼球屈光度、眼轴长度及曲率半径,第8周时对三组豚鼠行电生理闪光视网膜电图(F-ERG)检查,取出眼球后行病理评估组织学改变。结果 3组豚鼠眼球屈光度与眼轴长度呈正相关,第8周时与对照组相比,20 Hz正弦波频闪组及20 Hz方波屈光度出现(-0.75±0.79)D及(-1.50±0.91)D近视性改变(P<0.05),曲率半径3组间差异无显著性(P>0.05),F-ERG潜伏期20 Hz正弦波及20 Hz方波频闪组a波延长了3.8 s及7.9 s,病理三组间组织学未见显著性差异。结论持续暴露于高频方波及正弦波频闪光一定程度上影响眼正视化。
Objective To investigate the effect of prolonged stroboscopic illumination exposure on the growth of eyeball of guinea pig. Methods Thirty 2-week-old guinea pigs were randomized into three groups（ n = 10 for each）. Two strobe-reared groups were raised with 20 Hz sinusoidal and 20 Hz square wave stroboscopic illumination,respectively. The control group received usual light illumination. The illumination intensity was 500 lux. All animals underwent refraction and biometric measurements prior to and after 2,4,6 and 8 weeks of treatment. Finally,flash electroretinograms were compared,and retinal microstructures were examined. Results There was a significant correlation between refractive errors and axial eye elongation,and myopia increasing was observed with eye elongation. After 8 weeks of treatment,the animals raised in 20 Hz sinusoidal and 20 Hz square wave stroboscopic illumination were（-0. 75 ± 0. 79） D and（-1. 50 ± 0. 91）D more myopic than the group raised in continuous illumination. The implicit time of the a-wave was delayed by 3. 8 and7. 9 ms,respectively. No significant difference was found in retinal ultrastructures among the three groups. Conclusions Chronic exposure to 20 Hz sinusoidal or square wave stroboscopic illumination alters the emmetropization of the guinea pig eye to some extent.
出处
《中国实验动物学报》
CSCD
北大核心
2017年第5期500-505,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金(No.81400428)
关键词
频闪
频率
豚鼠
近视
屈光
Stroboscopic illumination
Frequency
Guinea pig
Myopia
Refraction
