摘要
解剖了三龄幼虫,获取唾腺体是果蝇唾腺染色体制片和观察的第一步。在长期的实验准备中,虽提供大量的三龄幼虫,但在解剖幼虫时因切入点不精确,且操作工具不当,最后较难得到理想的唾腺体,影响后期唾腺的染色体制片和观察。经过大量的实验,对解剖幼虫时的切入点和操作工具以及操作角度进行探索和改进。最终证明,改进后的方法不但能够节约时间,节省材料,而且能够快速地获得完整的唾腺体,大大提高染色体制片和观察的效果。
Anatomy of third-instar larvae,obtaining salivary glands,is the first step in the preparation and observa-tion of the Drosophila salivary gland chromosomes. In the long-term experimental preparation,although a large num-ber of third-instar larvae are provided,inaccuracies in the dissection of larvae and inaccurate handling tools make itharder to obtain ideal salivary glands,chromosomal preparations that affect later salivary glands and observed. Aftera large number of experiments,the larvae dissecting points and operating tools and operating point of exploration andimprovement. Ultimately,the improved method not only saves time,saves materials,but also obtains the complete sal-ivary gland quickly and greatly improves the effect of chromosome preparation and observation.
出处
《安徽农学通报》
2017年第21期39-40,63,共3页
Anhui Agricultural Science Bulletin
关键词
果蝇唾腺
解剖技巧
遗传学实验
Salivarygland
Anatomytechniques
Geneticsexperiment
