摘要
目的探讨β-内啡肽(β-EP)对溃疡性结肠炎(UC)患者免疫机制的调节作用。方法 UC患者30例,轻度活动期8例,中度活动期10例,重度活动期12例。同期结肠息肉患者15例为对照组。采用放射免疫法检测血液中β-EP、白细胞介素1β(IL-1β)的表达水平,同时应用免疫组织化学染色方法检测结肠黏膜组织中β-EP、IL-1β的表达水平;采用流式细胞术对血浆中的T细胞亚群(CD3^+、CD4^+、CD4^+/CD8^+)表达进行检测。结果病例组血液中β-EP、IL-1β、CD4^+、CD4^+/CD8^+各项指标的表达水平均明显高于对照组(P<0.05);且随病情严重程度不同,其表达水平也有差异,依次为:轻度活动组<中度活动组<重度活动组(P<0.05)。血浆中β-EP分别在活动期和缓解期的变化与IL-1β、CD4^+、CD4^+/CD8^+的变化呈正相关(r=0.522,P<0.01;r=0.532,P<0.05;r=0.964,P<0.01)。结论β-EP通过调节T细胞亚群及IL-1β的分泌参与了UC免疫机制的调节,在一定程度上可以反映UC患者病情的严重程度。
Objective To explore the immune mechanism of β-endorphin （β-EP） in ulcerative colitis （UC）patients. Methods Thirty UC patients were enrolled,including mild activity 8 cases,moderate activty 10 cases andsevere activity 12 cases. In the same period） 15 patients with colon polyps served as continterleukin-1 β（L-1β） were measured by radioimmunoassay. After peripheral blood lymphocyte separation, CD3＋ T cells,CI〉1＋ Tcclls and CD4＋/CD8＋ T cells percentage were detected by f 1 ow cytometry. β-EP and IL-1β in colonmucosa were measured by immunohistochcmistry. Results Plasma expressions of β-EP,IL-1β, CD4＋ T cells, CD4＋/ CD8＋ Tcclls percentage in patientswith UC were significantlyhighcr than those of control group （ P 〈 0. 05）. And the expressions showed significant difference with the disease severity： patients with mild UC〈paticnts with moderate UC〈paticnts with severe UC （ P 〈0. 05）. Colonic expressions of p-EP,IL-1β in patients with UC were significantlyhigher than those in control group （ P 〈 0.05）. Plasma p-EP between the active phase and the remission phase waspositively correlated with the change of IL-lp, CD4＋ T cells, CD4＋ /CD8＋ T cells percentage（ r = 0. 522,P 〈 0 . 01 ; r =0. 532, P 〈 0. 05 ; r = 0. 961,P〈0.01）. Conclusion p-EP may play a role in the immunological mechanism of UC by regulating the lcukomonocytc subgroup and the secretion of IL-1β in a certain way？p-EP reflects the severity of UC patients.
作者
刘琳
刘丽
张蕊
赵东强
刘学臣
张迪
Liu Lin Liu Li Zhang Rui Zhao Dongqia(Department of Gastroenterology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000 China Department of Gastroenterology,Handan Central Hospital,Handan 0b6000,China)
出处
《临床荟萃》
CAS
2017年第11期969-973,共5页
Clinical Focus
