摘要
目的 为了确定 1996年和 1998年沈阳地区发生的严重流行性感冒的病原 ,建立快速、敏感的诊断流感的方法。方法 用反转录 -聚合酶链反应 (RT -PCR)法 ,对门诊疑似流感病人咽拭子标本进行狗肾传代细胞 (MDCK)短时培养后流感病毒基因检测。结果 1996年流感流行期甲型流感病毒阳性率为 6 0 % ;1998年流感流行时甲型流感病毒阳性率为 83% ,说明 1996年和 1998年流感大流行皆为甲型流感病毒所致。结论 研究显示短时细胞培养后检测咽拭子标本中的流感病毒基因 。
Objective To determin the pathogen of serious epidemic of influenza occurred in Shenyang area in1996 and 1998,and for establishing rapid and sensitive method of diagnosis of influenza.Methods Throat swabs were collected from the patients who were suspected to have influenza,and influenza virus gene was detected by reverse transcription-polymerase chain reaction(RT-PCT)after short culture in MDCK cell.Results Positive rate of influenza virus A was 60% in 1996 and 83% in 1998.Influenza virus A was considered to be the pathogenic agent of epidemic in 1996 and 1998.Conclusion RT-PCR method after short cell culture was the best way for diagnosis of influenza.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第9期1113-1114,共2页
Chinese Journal of Public Health
