摘要
目的 在基因分型基础上探讨不同基因型别的幽门螺杆菌 (Hp)与疾病之间的关系。方法 采用随机扩增的多态性DNA(RAPD)和PCR -RFLP法对 5 0株来自不同病人的Hp进行分析 ,在此基础上运用聚类分析进行基因分型 ,并用病例对照研究探讨不同基因型别的Hp与疾病之间的关系。 结果 RAPD结果显示 ,不同基因型别的Hp与不同的疾病结局有关。PCR -RFLP结果显示 ,单个基因的分析结果均未能说明不同基因型别的Hp与不同的疾病结局有关 ,而多个基因的综合分析结果则说明不同基因型别的Hp确实与不同的疾病结局有关。将RAPD和PCR -RFLP的分析结果进行综合分析 ,也从多方法水平说明不同基因型别的Hp确实与不同的疾病结局有关。结论 对于Hp来说 ,多基因性状数值分类法的分型效率高于单基因数值分类法 ,这为建立Hp的多基因。
Objective To explore the relationship between the genetic types of helicobacter pylori and diseases.Methods Random amplification polymorphic DNA(RAPD) method and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method were used to analyze 50 isolates of Hp from different patients,then the cluster analysis was used to classify these isolates and a case-control study was used to explore the relationship between the genetic types of helicobacter pylori and different diseases.Results The results of RAPD showed that the genetic types of helicobacter pylori had the relationship with different gastrointestinal diseases.The results of PCR-RFLP can't show this relationship in the level of a single gene,but this relationship was also showed in the level of multiple genes.The results of RAPD and FCR-RFLP were combined to be analysed,which indicated that the genetic types of helicobacter pylori had the relationship with different outcome of diseases in the level of the multiple methods.Conclusion The numerical classification of the multiple gene was better than the typing method of a single gene.This would become the basis of building numerical typing system of Hp using multiple genes and multiple methods.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第9期1037-1039,共3页
Chinese Journal of Public Health
基金
河南省医学科技创新人才基金 (No 2 0 0 0 - 84)
河南省分子医学重点学科开放实验室资助项目
关键词
幽门螺杆菌
分子分型
分子流行病学
RAPD
PCR
RFLP
helicobacter pylori
molecular type
molecular epidemiology
