摘要
目的研究香青兰总黄酮对载脂蛋白E基因缺陷小鼠动脉粥样硬化病变的作用及可能的机制。方法载脂蛋白E基因缺陷小鼠随机分为模型组、辛伐他汀组及香青兰总黄酮高、中、低剂量组,以C57BL/6J小鼠为正常对照组,给药第12周后处死,检测血清血脂水平,主动脉粥样硬化斑块面积/管腔面积比值,免疫组织化学染色观察主动脉壁细胞间黏附分子1、血管细胞黏附分子1及增殖细胞核抗原的表达。结果与模型组比较,各给药组可以显著降低血清甘油三酯、低密度脂蛋白胆固醇水平(P<0.05或P<0.01);苏木素伊红染色观察给药组动脉粥样硬化病变减轻,斑块面积/管腔面积比值减小(P<0.05或P<0.01);免疫组织化学染色显示给药组细胞间黏附分子1、血管细胞黏附分子1及增殖细胞核抗原的表达下调(P<0.05或P<0.01)。结论香青兰总黄酮可能通过调节载脂蛋白E基因缺陷小鼠血脂代谢及下调主动脉壁细胞间黏附分子1、血管细胞黏附分子1及增殖细胞核抗原表达等的共同作用与影响,阻止载脂蛋白E基因缺陷小鼠动脉粥样硬化病变形成的发生、发展。
Aim To investigate the effect and possible mechanism of total flavones of dracocephalum moldavica （TFDM） on atherosclerosis lesions in apolipoprotein E gene deficient （ApoE^-/-） mice. Methods ApoE^-/- mice were ran- domly divided into model group, simvastatin group and TFDM high, medium, low dose group, C57BL/6J mice were set as the normal control group. After treated for 12 weeks, the mice were sacrificed and plasma lipids were determined, the area of ather- osclerotic plaques and the ratio of plaque area to aorta were measured by HE staining, the expressions of intercellular cell adhe- sion molecule- 1 （ICAM- 1）, vascular cell adhesion molecule- 1 （VCAM- 1）, proliferating cell nuclear antigen （PCNA） in aortic atherosclerotic lesions were detected by immunohistochemistry analysis. Results Compared with the model group, the levels of triglyceride （TG） and low density lipoprotein cholesterol （LDLC） was decreased significantly in treatment group. The athero- sclerotic lesions reduced and the ratio of plaque area to aorta decreased in TFDM groups. The expression of ICAM-1, VCAM-1 and PCNA were down-regulated by TFDM. Conclusion TFDM can inhibit atherosclerosis formation which involved in de- creasing the level of plasma lipids and weakening the expression of ICAM-1, VCAM-1 and PCNA in the aorta wall.
作者
全翊宁
王新春
郭新红
袁勇
曹文疆
程江
QUAN Yi-Ningl WANG Xin-Chun GUO Xin-Hong YUAN Yong CAO Wen-Jiang CHENG Jiang(The First Affiliated Hospital of Medical College, Shihezi University, Shihezi, Xinjiang 832008, China Medical College of Shihezi University, Shihezi , Xinjiang 832002, China)
出处
《中国动脉硬化杂志》
CAS
北大核心
2017年第9期874-878,共5页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金项目(81460638
81660704
81360631)
关键词
香青兰总黄酮
动脉粥样硬化
细胞间黏附分子1
血管细胞黏附分子1
增殖细胞核抗原
Total flavones of dracocephalum moldavica
Atherosclerosis
Intercellular cell adhesion molecule-1
Vascular cell adhesion molecule-1
Proliferating cell nuclear antigen
