摘要
目的:研究余甘子鞣质部位主要药效成分在人工模拟胃、肠液中的稳定性,为余甘子体内代谢研究提供依据。方法:采用人工模拟胃液、肠液,体外温孵,HPLC-UV法测定余甘子鞣质部位中主要药效成分的含量变化情况;分别考察余甘子鞣质部位主要药效成分在人工胃肠液中的稳定性。结果:余甘子鞣质部位中主要药效成分没食子酸、柯里拉京、鞣花酸在人工胃液中比较稳定,含量变化不明显,降解剩余量在100%左右波动,半衰期大于90 h;在人工肠液中不稳定,含量先增加后减少,降解剩余量在100-300%之间波动,半衰期大于10 h。结论:余甘子鞣质部位主要药效成分在人工胃液中比较稳定,各成分含量变化不明显;余甘子鞣质部位主要药效成分在人工肠液中不稳定,主要成分含量先增加后减少,推测大分子可水解鞣质可以转化成小分子成分,但是随着时间延长所有成分均在降解。
This article was aimed to study the stability of tannin parts in Phyllanthus emblica L. in artificial gastric and intestinal juice, in order to provide a foundation for in vivo studies of Phyllanthus emblica L. HPLC-UV was used to determine the contents of main ingredients. The results showed that stability of main ingredients of tannin parts in Phyllanthus emblica L., including gallic acid （GA）, corilagin and ellagic acid （EA）, in the artificial gastric juice. The content change was not obvious. The residual content was within 100%. The half-life was above 90 h. They were unstable in the artificial intestinal juice. The content was first increased and then decreased. The residual content was 100-300%.The half-life was above 10 h. It was concluded that the tannin parts in Phyllanthus emblica L. was stable in artificial gastric juice. And content changes of main components were not obvious. The tannin parts in Phyllanthus emblica L. were unstable in artificial intestinal juice. The main ingredients were first increased and then decreased. It was speculated that macromolecule can hydrolyze tannin part into small molecules. However, as time increases, all components may be decomposed.
出处
《世界科学技术-中医药现代化》
CSCD
2017年第8期1386-1390,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金面上项目(81274187):基于藏药余甘子酚酸类成分体内动态变化的的抗癌药效物质与质量控制
负责人:张兰珍
关键词
余甘子
鞣质部位
人工胃
肠液
稳定性
Phyllanthus emblica L., tannin part, artificial gastric and intestinal juice, stability
