摘要
目的通过蛋白质组学技术,筛选乳腺癌患者和健康人血清的差异表达蛋白,以期筛查乳腺癌早期诊断的肿瘤标志物。方法收集乳腺癌患者血清10例、健康人血清各10例,采用双向电泳(2-DE)技术分离蛋白,通过PDQuest软件筛选乳腺癌和健康人血清表达差异的蛋白样点,进一步质谱分析鉴定。结果初步建立人乳腺癌、健康人血清双向电泳图谱,PDQuest软件分析2-DE电泳结果,检测出22个差异蛋白点,以健康人组为参照,乳腺癌组上调有18个蛋白样点,下调有4个。从22个差异点中筛选其中12个差异蛋白样点进行取样、胶内酶切、质谱鉴定,成功鉴定4个表达差异的蛋白点,通过数据库检索最终确定上述蛋白点对应的蛋白质分别为锚蛋白-3亚型X7(ankyrin-3 isoform X7)、keratin,type I cyto-skeletal 9、核膜血影重复蛋白2亚型X11(nesprin-2 isoform X11)、Dynein heavy chain 10,axonemal iso-form X9。结论健康人组和乳腺癌组筛选出的差异蛋白质可能成为乳腺癌的早期诊断标志物。
Objective To screen tumor biomarker of breast cancer from expressed differently in breastcancer patients and health by proteomics techniques. Methods Collecting 10 serum cases of breast cancer andhealthy respectively,protein spot was separated by two-dimensional electrophoresis,protein expressed differentlyfrom breast cancer and healthy was screened by PDQuest software,and identified by mass spectrometry. Results The 2-DE maps of breast cancer and healthy were established,22 differentially expressed proteins were analyzedby PDQuest software,took the healthy group as the reference,there were 18 spots with up-regulated serum protein,and 4 spots with down-regulated serum proteins in breast cancer group.12 protein spots were sampled and detectedby mass spectrometry. Finally,4 differentially expressed proteins were identified successfully,the proteins wereconfirmed by the database,named as ankyrin-3 isoform X7,keratin,type I cytoskeletal 9,nesprin-2 isoform X11,Dynein heavy chain 10,axonemal isoform X9. Conclusion The 4 differentially expressed proteins in healthygroup and breast cancer group can be potential candidate biomarkers for diagnosis of breast cancer.
出处
《实用医学杂志》
CAS
北大核心
2017年第20期3354-3358,共5页
The Journal of Practical Medicine
基金
四川省科技厅-泸州市政府-泸州医学院联合课题(编号:14JC0155)
泸州市科技局项目[编号:2012-S-36(3/14)]
