摘要
目的:研究白细胞介素10(IL-10)对miRNA-125b表达和非小细胞肺癌增殖与凋亡的影响。方法:采用实时荧光定量PCR检测IL-10刺激肺癌细胞系NCI-H460各微小RNA(miRNA)的表达水平变化;利用细胞计数试剂盒(CCK8)比较IL-10刺激后和恢复miRNA-125b表达后对细胞增殖的影响;流式细胞术检测IL-10刺激后和恢复miRNA-125b表达后对细胞凋亡的影响;荧光定量PCR技术验证miRNA-125b在非小细胞肺癌组织和癌旁组织中的表达情况。结果:IL-10刺激NCI-H460细胞后,miRNA受到调控的程度不同,所测21种miRNA中,miRNA-125b下调程度最大;miRNA-125b恢复表达后,抑制了由IL-10刺激引起的细胞增殖(P<0.01),促进了细胞凋亡;miRNA-125b在非小细胞肺癌组织中的表达显著低于癌旁组织(P<0.01)。结论:在IL-10诱导的细胞增殖中,miRNA-125b起到抑癌的作用,可作为未来治疗非小细胞肺癌的药物研发对象。
Objective：To investigate the interleukin-10（IL-10） on the expression of miRNA-125b,and cell proliferation and apoptosis in non-small cell lung cancer. Methods：The expression level of miRNAs in H460 cells stimulated by IL-10 was detected using real-time fluorescent quantitative PCR. The effects on cell proliferation between IL-10 stimulating and recovery of miRNA-125b expression were compared using CCK8 method. The effects of IL-10 stimulating and recovery of miRNA-125b expression on cell apoptosis were evaluated by flow cytometry. The expressions of miRNA-125b in non-small cell lung cancer and adjacent tissues were verified by real-time fluorescent quantitative PCR. Results：There was different degree expressions of 21 kinds of miRNAs in H460 cells stimulated by IL-10, and the expression decreasing of miRNA-125b was the maximum. After the expression of miRNA-125b recovering,the proliferation and apoptosis of cell stimulated by IL-10 was inhibited and promoted,respectively（P〈0. 05）. The expression of miRNA-125b in non-small cell lung cancer tissue was significantly lower than that in normal tissue（P〈0. 01）. Conclusions：During the cell proliferation induced by IL-10,miRNA-125b can play a role on inhibiting cancer,which is expected to be used as a drug development target in the treatment of non-small cell lung cancer.
作者
胡嵩
张岩巍
孙贝贝
HU Song ZHANG Yan-wei SUN Bei-bei(Department of Research Cancer Department of Pulmonary, Shanghai Chest Hospital Affiliated to Shanghai JiaoTong University, Shanghai 200030, China)
出处
《蚌埠医学院学报》
CAS
2017年第7期848-851,共4页
Journal of Bengbu Medical College
基金
CSCO-先声抗肿瘤血管靶向治疗科研基金(Y-S2014-003)
关键词
非小细胞肺癌
白细胞介素-10
增殖
凋亡
miRNA-125b
non-small cell lung cancer
miRNA-125b
proliferation
apoptosis
