摘要
目的建立能同时检测化妆品中10种α-羟基酸的高效液相色谱方法。方法对色谱柱类型、流动相组成、流动相p H值、流动相浓度、检测波长和流速等条件进行了系统优化。样品用水超声提取20 min,离心、过滤,以资深堂ADME C18色谱柱分离分析,以甲醇-0.1 mol/L的磷酸氢二铵溶液(磷酸调p H=2.45)为流动相进行梯度洗脱,进样量5μl,二极管阵列检测器检测,检测波长为214 nm,结合保留时间和光谱图定性分析,外标法定量。结果该方法前处理简单,线性范围宽,检出限(LOD)为2μg/g^400μg/g,回收率为85%~115%,相对标准偏差(RSD)为0.2%~6.8%。结论该方法具有可靠的准确度和精密度,可用于化妆品中亚酒石酸、乳酸、酒石酸、乙醇酸、苹果酸、柠檬酸、丙酮酸、2-羟基丁酸、羟基辛酸和扁桃酸10种α-羟基酸含量的检测。
Objective To develop a method for the simultaneous determination of 10 α-hydroxy acids in cosmetics by high performance liquid chromatography（ HPLC）. Methods The sample was extracted with water for 20 min ultrasonically. After being centrifuged and filtrated,CAPCELL PAK ADME-C18 column was employed in gradient elution mode using methyl alcohol-0. 1 mol/L（ NH4）2HPO4（ p H = 2. 45） as the mobile phase for gradient elution,with the feeding volume of 5 μl. The qualification analysis was done by using retention time and DAD spectrum and the detective wavelength was 214 nm. External standard method was used for quantification. Results The pretreating method was simple and had a good linear range. The limit of detections（ LODs） was within 2 μg/g-400 μg/g,the recoveries were within 85%-115%,and the relative standard deviations（ RSDs） was within 0. 2%-6. 8%. Conclusion The results indicated that the method is accurate and suitable for the determination of 10 α-hydroxy acids including a-hydroxymalonic acid,tartaric acid,glycolic acid,pyruvic acid,malic acid,lactic acid,citric acid,mandelic acid,hydroxycaprylic acid and 2-hydroxy-butanoic acid in cosmetics samples.
出处
《中国卫生检验杂志》
CAS
2017年第19期2757-2760,共4页
Chinese Journal of Health Laboratory Technology
关键词
化妆品
Α-羟基酸
高效液相色谱法
Cosmetics
α-hydroxy acid
High performance liquid chromatography
