摘要
目的 :探讨丹参和 β -七叶皂甙钠对烧伤后急性肺损伤的治疗作用及其可能机制。 方法 :采用大鼠 30 %TBSAⅢ度烫伤模型 ,将 4 5只大鼠随机分为假烫组、盐水组、丹参组、β -七叶皂甙钠组及联合组 ,烫伤后 2 4h取静脉血测外周血白细胞粘附聚集 (LAA) ,取肺组织检测髓过氧化物酶 (MPO)、丙二醛 (MDA)、超氧化物歧化酶 (SOD)含量。 结果 :丹参组、β -七叶皂甙钠组及联合组外周血LAA、肺组织MPO及MDA均低于盐水组 ,联合组最低。丹参组、β -七叶皂甙钠组及联合组SOD较盐水组明显增高 ,联合组最高。外周血LAA与肺组织MPO含量呈正相关。 结论 :丹参及 β -七叶皂甙钠均可减轻中性粒细胞 (PMN)在肺内的聚集、粘附 ,减轻氧自由基 (OFR)及其代谢产物对肺组织的损伤 ,增强SOD对机体的保护作用 ,联合应用效果更佳。
Objective:To evaluate the therapeutic mechanism and effects of Salvias Miltiorrhizae and β-Aescinom Natricum on acute lung damage following thermal injury Methods:All the rats except those in the sham injury group were inflicted with 30% total body surface area(TBSA)third degree burns.Forty five rats were involved in this study.They were divided into five groups:sham injury group(n=9),saline group(n=9),Salviae Miltiorrhizae group(n=9),β-Aescinom Natricum group (n=9)and combination group(n=9).The rats were sacrificed 24 hours after injury.The status of leucocyte sdhesiveness/aggregation(LAA)in peripheral blood,and myeloperoxidase(MPO),malondialdehyde(NDA),superoxide dismutase(SOD)contents in lung tissue were determined.Results:A significant increase in blood LAA,tissue MPO,and MDA contents was found in Salviae Miltiorrhizae group,β-Aescinom Natricum group and combination group as compared with those in saline group,and those in the combination group were the lowest among the three groups.A significant decline in tissue SOD contents were found in Salviae Miltiorrhizae group,β-Aescinom Natricum group and combination group as compared with those in saline group,and those in the combination group were higher than the other two groups.Blood LAA was positively correlated with tissue MPO content.Conclusion:Both Salviae Miltiorrhiazae and β-Aescinom Natricum can decrease adhesiveness and aggregation of PMNs in lung tissue,inhibit oxygen free radical formation and increase the protective effect of SOD.The combination of the two drugs has protective effects on acute lung damage following thermal injury.
出处
《中国中西医结合外科杂志》
CAS
2002年第4期290-292,共3页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
