摘要
目的获取除磷菌纯菌株P7,研究其生长特性和除磷性能.方法从运行良好的生物除磷反应器中分离出一株高效除磷菌株P7,对该菌株进行除磷能力检测、生理生化鉴定、16S rRNA基因比对、生长曲线监测和除磷条件优化等.结果经鉴定菌株P7为假单胞菌,Genbank登记号为KX181642.在不同温度条件下菌株P7均能较好增殖,生长曲线出现了比较明显的迟缓期、对数期,适宜生长温度为25~35℃,最佳生长温度为35℃,当pH为6~8时,菌株P7的生长曲线比较典型,最佳生长pH值为7;菌株P7在不同温度条件下的磷酸盐去除率均在60%以上,最佳除磷温度为30℃,此时磷酸盐去除率达到最大值98.1%,最佳除磷pH值为7.结论菌株P7与其他除磷菌株相比每小时吸磷率最大、最大吸磷量均处于中上水平,除磷能力较高.
A high efficiency phosphorus removal bacteria pure strain P7 was isolated from the biological phosphorus removal reactor in laboratory. The strain P7 was Pseudomonas sp. after physiological and biochemical identification and 16 S rRNA gene comparison. Genbank registration number was KX181642. The effects of different temperature and pH on the growth characteristics of strain P7 showed that, strain P7 proliferated good under different temperature conditions. And the growth curve of strain P7 showed a lag phase and logarithmic phase obviously under suitable growth temperature range of 25 ℃ ~ 35 ℃. The suitable pH of strain P7 was 6 ~ 8. The phosphorus removal rate of strain P7 was studied under different temperature and pH conditions. The phosphorus removal rate of strain P7 was above 60%under different temperature conditions,and the maximum phosphorus removal rate was 98. 1%at 30 ℃and pH 7. In summary, the optimal growth temperature of strain P7 is 35 ℃, the optimum growth pH was 7, the best removal temperature was30 ℃,and the optimum pH was 7. Compared with other phosphorus removal bacteria, the phosphorus removal capacity of strain P7 was higher.
作者
亢涵
藏春月
焦洋
唐婧
李玲
KANG Han ZANG Chunyue JIAO Yang TANG JingI ZI Ling(School of Municipal and Environmental Engineering ,Shenyang Jianzhu University, Shenyang ,China 110168 School of Architecture and Urban Planning, Shenyang Jianzhu University,Shenyang ,China 110168)
出处
《沈阳建筑大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第5期945-952,共8页
Journal of Shenyang Jianzhu University：Natural Science
基金
国家自然科学基金项目(51308354)
辽宁省教育厅项目(LJZ2016003)
关键词
生物除磷
假单胞菌
生长曲线
除磷性能
biological phosphorus removal
pseudomonas sp
growth curve
phosphorus removal ability
