摘要
目的探讨前列腺素D2合成酶（PTGDS）在非小细胞肺癌（NSCLC）[包括肺腺癌（ADC）和肺鳞癌（SCC）]中的作用及可能的机制。方法首先，通过基因表达汇编（GEO）数据库对PTGDS在ADC和SCC中各自的蛋白表达进行分析。然后，通过组织芯片对PTGDS的蛋白表达进行验证。最后，选取A549和Calu-3细胞系过表达PTGDS，采用体外侵袭实验检测其侵袭能力，采用蛋白质印迹法进行检测其蛋白表达的变化。结果PTGDS在ADC和SCC中的蛋白表达均下调，但是在ADC和SCC之间差异无统计学意义，且其仅与ADC的肿瘤发展相关。在过表达PTGDS以后，ADC肿瘤细胞的侵袭能力显著下降，蛋白质印迹法显示其内部机制可能与MAPK信号通路有关。结论PTGDS在NSCLC中表达下调，其与ADC的发展有关，而与SCC的发展无关。PTGDS是NSCLC早期诊断与预后判断的一个潜在的肿瘤标志物，其是否可用于NSCLC的药物治疗将是我们接下来研究的重点。
Objective To investigate prostaglandin D2 synthase （PTGDS） expression in non-small cell lung carcinoma （NSCLC） and clarify whether PTGDS could be a biomarke. Methods Firstly, the protein expression level of PTGDS in adenocarcinoma （ADC） and squamous cell carcinoma （SCC） were analyzed respectively through Gene Expression Omnibus （GEO） dataset. Then, the results were verified by using tissue microarray （TMA）. At last, in order to explore the inner mechanism, lung adenocarcinoma cell lines A549 and Calu-3 were selected to over-express PTGDS, and then transwell chamber assay and Western blotting were used to detect associated changes. Results PTGDS expression is down-regulated in both ADC and SCC, however, no statistical difference has been found between the two groups, and PTGDS was only related to the progression of ADC. Furthermore, After over-expression of PTGDS, the invasiveness of ADC cells was significantly decreased. Western blotting showed that the inner mechanisms may be related to mitogen-activated protein kinase （MAPK） signal pathway. Conclusions This study revealed that PTGDS was down-regulated in NSCLC and only related to the development of ADC. It may be a potential biomarker for the diagnosis and prognosis of NSCLC. However, whether it could be used for the treatment of NSCLC still needs more research.
作者
贺利平
陈毅斐
杨炯
He Liping Chen Yifei Yang Jiong.(Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China)
出处
《中华医学杂志》
CAS
CSCD
北大核心
2017年第38期3022-3027,共6页
National Medical Journal of China
